@article{fdi:010055924,
  title = {{L}arge-scale species delimitation method for hyperdiverse groups},
  author = {{P}uillandre, {N}. and {M}odica, {M}. {V}. and {Z}hang, {Y}. and {S}irovich, {L}. and {B}oisselier, {M}. {C}. and {C}ruaud, {C}. and {H}olford, {M}. and {S}amadi, {S}arah},
  editor = {},
  language = {{ENG}},
  abstract = {{A}ccelerating the description of biodiversity is a major challenge as extinction rates increase. {I}ntegrative taxonomy combining molecular, morphological, ecological and geographical data is seen as the best route to reliably identify species. {C}lassic molluscan taxonomic methodology proposes primary species hypotheses ({PSH}s) based on shell morphology. {H}owever, in hyperdiverse groups, such as the molluscan family {T}urridae, where most of the species remain unknown and for which homoplasy and plasticity of morphological characters is common, shell-based {PSH}s can be arduous. {A} four-pronged approach was employed to generate robust species hypotheses of a 1000 specimen {S}outh-{W}est {P}acific {T}urridae data set in which: (i) analysis of {COI} {DNA} {B}arcode gene is coupled with (ii) species delimitation tools {GMYC} ({G}eneral {M}ixed {Y}ule {C}oalescence {M}ethod) and {ABGD} ({A}utomatic {B}arcode {G}ap {D}iscovery) to propose {PSH}s that are then (iii) visualized using {K}lee diagrams and (iv) evaluated with additional evidence, such as nuclear gene r{RNA} 28{S}, morphological characters, geographical and bathymetrical distribution to determine conclusive secondary species hypotheses ({SSH}s). {T}he integrative taxonomy approach applied identified 87 {T}urridae species, more than doubling the amount previously known in the {G}emmula genus. {I}n contrast to a predominantly shell-based morphological approach, which over the last 30 years proposed only 13 new species names for the {T}urridae genus {G}emmula, the integrative approach described here identified 27 novel species hypotheses not linked to available species names in the literature. {T}he formalized strategy applied here outlines an effective and reproducible protocol for large-scale species delimitation of hyperdiverse groups.},
  keywords = {{ABGD} method ; barcoding ; {C}onoidea ; {GMYC} method ; {K}lee diagrams ; {I}ntegrative taxonomy ; {T}urridae},
  booktitle = {},
  journal = {{M}olecular {E}cology},
  volume = {21},
  numero = {11},
  pages = {2671--2691},
  ISSN = {0962-1083},
  year = {2012},
  DOI = {10.1111/j.1365-294{X}.2012.05559.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010055924},
}