@article{fdi:010055875,
  title = {{A} simple protocol for cryopreservation of zygotic embryos of ten accessions of coconut ({C}ocos nucifera {L}.)},
  author = {{N}'{N}an, {O}ulo and {B}orges, {M}. and {K}onan, {J}. {L}. {K}. and {H}ocher, {V}al{\'e}rie and {V}erdeil, {J}. {L}. and {T}regear, {J}ames and {N}'{G}uetta, {A}. {S}. {P}. and {E}ngelmann, {F}lorent and {M}alaurie, {B}ernard},
  editor = {},
  language = {{ENG}},
  abstract = {{A} simple and efficient cryopreservation protocol for coconut zygotic embryos has been developed. {E}mbryos were inoculated in {P}etri dishes on medium containing 3.2 {M} glucose, which were placed in hermetically closed containers containing 80 or 160 g silica gel for 48 or 24 h. {M}oisture content of embryos at the end of this treatment varied between 0.25 and 0.65 g g(-1) {DW}, depending on the accession. {E}mbryos were then transferred for cryopreservation by rapid immersion of cryotubes in liquid nitrogen. {A}fter rapid re-warming, embryos were transferred to culture medium containing {E}euwens mineral elements for germination. {T}his protocol was applied to ten accessions representative of coconut genetic diversity, with germination percentages of cryopreserved embryos between 13.7% and 74.7%.},
  keywords = {{C}oconut '{D}warf' and '{T}all' types ; {G}enetic resources conservation ; {G}lucose ; {O}ne-step dehydration ; {T}ropical crop},
  booktitle = {},
  journal = {{I}n {V}itro {C}ellular and {D}evelopmental {B}iology : {P}lant},
  volume = {48},
  numero = {2},
  pages = {160--166},
  ISSN = {1054-5476},
  year = {2012},
  DOI = {10.1007/s11627-012-9425-4},
  URL = {https://www.documentation.ird.fr/hor/fdi:010055875},
}