@article{fdi:010055820,
  title = {{I}nitial proteome analysis of caffeine-induced proteins in {A}spergillus tamarii using two-dimensional fluorescence difference gel electrophoresis},
  author = {{G}utierrez-{S}anchez, {G}. and {A}twood, {J}. and {K}olli, {V}. {S}. {K}. and {R}oussos, {S}evastianos and {A}ugur, {C}hristopher},
  editor = {},
  language = {{ENG}},
  abstract = {{C}affeine is toxic to most microorganisms. {H}owever, some filamentous fungi, such as {A}spergillus tamarii, are able to metabolize this alkaloid when fed caffeine as the sole nitrogen source. {T}he aim of the present work was to identify intracellular {A}. tamarii proteins, regulated by caffeine, using fluorescence difference two-dimensional gel electrophoresis. {S}pecific proteins from two culture media of {A}. tamarii grown either on ammonium sulfate or caffeine as the sole nitrogen source were analysed by mass spectrometry. {T}hirteen out of a total of 85 differentially expressed spots were identified after database search. {I}dentified up-regulated proteins include phosphoglycerate kinase, malate dehydrogenase, dyp-type peroxidase family protein, heat shock protein, {C}u, {Z}n superoxidase dismutase and xanthine dehydrogenase. {S}ome of the proteins identified in this study are involved in the caffeine degradation pathway as well as in stress response, suggesting that stress proteins could be involved in caffeine metabolism in filamentous fungi.},
  keywords = {{T}wo-dimensional electrophoresis ; {D}ifference gel electrophoresis ; {P}roteomics ; {F}ilamentous fungi ; {C}affeine degradation ; {M}ass spectrometry},
  booktitle = {},
  journal = {{A}pplied {B}iochemistry and {B}iotechnology},
  volume = {166},
  numero = {8},
  pages = {2064--2077},
  ISSN = {0273-2289},
  year = {2012},
  DOI = {10.1007/s12010-012-9634-7},
  URL = {https://www.documentation.ird.fr/hor/fdi:010055820},
}