AguileraM.auteurautRakotoarivoninaH.auteurautBrutusA.auteurautGiardinaT.auteurautSimonGwenolaauteurautIRDFonsM.auteurauttextjournalArticletext/pdfborn digitalaccessDifferential gene expression analysis was performed in monoxenic mice colonized with Ruminococcus gnavus strain El, a major endogenous member of the gut microbiota. RNA arbitrarily primed-PCR fingerprinting assays allowed to specifically detect the in vivo expression of the agal gene, which was further confirmed by RT-PCR. The agal gene encoded a protein of 744 residues with calculated molecular mass of 85,207 Da. Agal exhibited significant similarity with previously characterized alpha-Galactosidases of the GH 36 family. Purified recombinant protein demonstrated high catalytic activity (104 +/- 7 U mg(-1)) and efficient p-nitrophenyl-alpha-D-galactopyranoside hydrolysis [k(cat)/K-m = 35.115 +/- 8.82 s(-1) mM(-1) at 55 degrees C and k(cat)/K-m = 17.48 +/- 4.25 s(-1) mM(-1) at 37 degrees C].specializedGut microbiotaRuminococcus gnavusAlpha-GalactosidaseIn vivo expressionmRNA fingerprintsFRANCE084 1631142120120923-2508http://www.documentation.ird.fr/hor/fdi:01005565210.1016/j.resmic.2011.10.005IRD BondyF B010055652http://www.documentation.ird.fr/hor/fdi:010055652http://www.documentation.ird.fr/intranet/publi/2012/03/010055652.pdfAccès réservé (Intranet de l'IRD)IRD - Base Horizon / Pleins textes2012-04-042012-09-12fdi:010055652fre