@article{fdi:010055652,
  title = {{A}ga1, the first alpha-galactosidase from the human bacteria {R}uminococcus gnavus {E}1, efficiently transcribed in gut conditions},
  author = {{A}guilera, {M}. and {R}akotoarivonina, {H}. and {B}rutus, {A}. and {G}iardina, {T}. and {S}imon, {G}wenola and {F}ons, {M}.},
  abstract = {{D}ifferential gene expression analysis was performed in monoxenic mice colonized with {R}uminococcus gnavus strain {E}l, a major endogenous member of the gut microbiota. {RNA} arbitrarily primed-{PCR} fingerprinting assays allowed to specifically detect the in vivo expression of the agal gene, which was further confirmed by {RT}-{PCR}. {T}he agal gene encoded a protein of 744 residues with calculated molecular mass of 85,207 {D}a. {A}gal exhibited significant similarity with previously characterized alpha-{G}alactosidases of the {GH} 36 family. {P}urified recombinant protein demonstrated high catalytic activity (104 +/- 7 {U} mg(-1)) and efficient p-nitrophenyl-alpha-{D}-galactopyranoside hydrolysis [k(cat)/{K}-m = 35.115 +/- 8.82 s(-1) m{M}(-1) at 55 degrees {C} and k(cat)/{K}-m = 17.48 +/- 4.25 s(-1) m{M}(-1) at 37 degrees {C}].},
  keywords = {{G}ut microbiota ; {R}uminococcus gnavus ; {A}lpha-{G}alactosidase ; {I}n vivo expression ; m{RNA} fingerprints ; {FRANCE}},
  journal = {{R}esearch in {M}icrobiology},
  volume = {163},
  numero = {1},
  pages = {14--21},
  ISSN = {0923-2508},
  year = {2012},
  DOI = {10.1016/j.resmic.2011.10.005},
  URL = {http://www.documentation.ird.fr/hor/fdi:010055652},
}