Suitability of an open automated nucleic acid extractor for high-throughput plasma HIV-1 RNA quantitation in Gabon (Central Africa)LiégeoisFlorianauteurautIRDBouéV.auteurautMouinga-OndéméA.auteurautLékanéD.K.auteurautMongoD.auteurautSicaJ.auteurautRouetF.auteurauttextjournalArticletext/pdfborn digitalaccessNucleic acid extraction using the open automated EZ1 (Qiagen) instrument, in combination with the Generic HIV Viral Load assay, gave highly concordant HIV-1 RNA viral load results among 181 Gabonese subjects infected with HIV-1, compared to those obtained when performing a manual extraction. Since people living with HIV-1 are being treated with antiretrovirals in Gabon, this automated extraction technique represents an excellent technical method for high-throughput monitoring of HIV-1 RNA viral load.specializedHIV-1Viral loadNucleic acid extractionAfricaGABON052 Journal of Virological Methods179126927120120166-0934http://www.documentation.ird.fr/hor/fdi:01005441510.1016/j.jviromet.2011.10.001IRD BondyF B010054415http://www.documentation.ird.fr/hor/fdi:010054415http://www.documentation.ird.fr/intranet/publi/2012/03/010054415.pdfAccès réservé (Intranet de l'IRD)IRD - Base Horizon / Pleins textes2012-04-042012-09-12fdi:010054415fre