@article{fdi:010054413, title = {{SSR} markers in transcripts of genes linked to post-transcriptional and transcriptional regulatory functions during vegetative and reproductive development of {E}laeis guineensis}, author = {{T}ranbarger, {T}imothy and {K}luabmongkol, {W}. and {S}angsrakru, {D}. and {M}orcillo, {F}. and {T}regear, {J}ames and {T}ragoonrung, {S}. and {B}illotte, {N}.}, editor = {}, language = {{ENG}}, abstract = {{B}ackground: {T}he oil palm ({E}laeis guineensis {J}acq.) is a perennial monocotyledonous tropical crop species that is now the world's number one source of edible vegetable oil, and the richest dietary source of provitamin {A}. {W}hile new elite genotypes from traditional breeding programs provide steady yield increases, the long selection cycle (10-12 years) and the large areas required to cultivate oil palm make genetic improvement slow and labor intensive. {M}olecular breeding programs have the potential to make significant impacts on the rate of genetic improvement but the limited molecular resources, in particular the lack of molecular markers for agronomic traits of interest, restrict the application of molecular breeding schemes for oil palm. {R}esults: {I}n the current study, 6,103 non-redundant {EST}s derived from c{DNA} libraries of developing vegetative and reproductive tissues were annotated and searched for simple sequence repeats ({SSR}s). {P}rimer pairs from sequences flanking 289 {EST}-{SSR}s were tested to detect polymorphisms in elite breeding parents and their crosses. 230 of these amplified {PCR} products, 88 of which were polymorphic within the breeding material tested. {A} detailed analysis and annotation of the {EST}-{SSR}s revealed the locations of the polymorphisms within the transcripts, and that the main functional category was related to transcription and post-transcriptional regulation. {I}ndeed, {SSR} polymorphisms were found in sequences encoding {AP}2-like, b{ZIP}, zinc finger, {MADS}-box, and {NAC}-like transcription factors in addition to other transcriptional regulatory proteins and several {RNA} interacting proteins. {C}onclusions: {T}he identification of new {EST}-{SSR}s that detect polymorphisms in elite breeding material provides tools for molecular breeding strategies. {T}he identification of {SSR}s within transcripts, in particular those that encode proteins involved in transcriptional and post-transcriptional regulation, will allow insight into the functional roles of these proteins by studying the phenotypic traits that cosegregate with these markers. {F}inally, the oil palm {EST}-{SSR}s derived from vegetative and reproductive development will be useful for studies on the evolution of the functional diversity within the palm family.}, keywords = {{THAILANDE} ; {BENIN}}, booktitle = {}, journal = {{B}mc {P}lant {B}iology}, volume = {12}, numero = {}, pages = {1}, ISSN = {1471-2229}, year = {2012}, DOI = {10.1186/1471-2229-12-1}, URL = {https://www.documentation.ird.fr/hor/fdi:010054413}, }