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Fki L., Bouaziz N., Kriaa W., Benjemaa Masmoudi R., Gargouri Bouzid R., Rival Alain, Drira N. Multiple bud cultures of 'Barhee' date palm (Phoenix dactylifera) and physiological status of regenerated plants. Journal of Plant Physiology, 2011, 168 (14), p. 1694-1700. ISSN 0176-1617
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doi:10.1016/j.jplph.2011.03.013
| Titre | Multiple bud cultures of 'Barhee' date palm (Phoenix dactylifera) and physiological status of regenerated plants |
| Année de publication | 2011 |
| Type de document | Article référencé dans le Web of Science WOS:000294832300011 |
| Auteurs | Fki L., Bouaziz N., Kriaa W., Benjemaa Masmoudi R., Gargouri Bouzid R., Rival Alain, Drira N. |
| Source | Journal of Plant Physiology, 2011, 168 (14), p. 1694-1700. ISSN 0176-1617 |
| Résumé | Adventitious bud clusters of date palm 'Barhee' were successfully established from juvenile leaves (<1 cm) using reduced amounts of 2,4-D (0.2 mg L-1) to limit the risk of somaclonal variation. An average of 8.4 adventitious buds per explant were obtained. Histological examination showed that the superficial cell layers of leaves had the highest caulogenic capacity. High sucrose concentration (70 g L-1) was used for the conversion of initial buds to multiple bud clusters. The promoting effect of temporary immersion on shoot proliferation was found to be significant when compared to cultivation on solid media. Elongation of shoots was also better using a thin film of PGR-free liquid medium instead of a solid medium. Anatomical observations indicated that roots from vitroplants were potentially functional at various developmental stages. However, only 12-month-old vitroplants were found to be physiologically able to control transpirational vapor loss. Additionally, the photochemical activity of photosystem II in these vitroplants was close to that measured in plants that were already acclimatized. As a result. 83.3% of regenerated plants were successfully acclimatized. No phenotypic variation was observed among more than 500 adventitious bud-derived plants. All regenerants survived after field transplantation. We found that the production of adventitious bud clusters in small bioreactors was able to provide an efficient micropropagation system for date palm cv. `Barhee'. An in vitro hardening step was a prerequisite for the successful transfer of vitroplants in soil. |
| Plan de classement | 076 |
| Localisation | Fonds IRD [F B010053828] |
| Identifiant IRD | fdi:010053828 |
| Lien permanent | http://www.documentation.ird.fr/hor/fdi:010053828 |
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