Vidal-Dupiol J., Ladrière O., Destoumieux Garzon D., Sautière P. E., Meistertzheim A. L., Tambutté E., Tambutté S., Duval D., Fouré L., Adjeroud Mehdi, Mitta G. (2011). Innate immune responses of a scleractinian coral to vibriosis. Journal of Biological Chemistry, 286 (25), p. 22688-22698. ISSN 0021-9258.
Titre du document
Innate immune responses of a scleractinian coral to vibriosis
Année de publication
2011
Auteurs
Vidal-Dupiol J., Ladrière O., Destoumieux Garzon D., Sautière P. E., Meistertzheim A. L., Tambutté E., Tambutté S., Duval D., Fouré L., Adjeroud Mehdi, Mitta G.
Source
Journal of Biological Chemistry, 2011,
286 (25), p. 22688-22698 ISSN 0021-9258
Scleractinian corals are the most basal eumetazoan taxon and provide the biological and physical framework for coral reefs, which are among the most diverse of all ecosystems. Over the past three decades and coincident with climate change, these phototrophic symbiotic organisms have been subject to increasingly frequent and severe diseases, which are now geographically widespread and a major threat to these ecosystems. Although coral immunity has been the subject of increasing study, the available information remains fragmentary, especially with respect to coral antimicrobial responses. In this study, we characterized damicornin from Pocillopora damicornis, the first scleractinian antimicrobial peptide (AMP) to be reported. We found that its precursor has a segmented organization comprising a signal peptide, an acidic proregion, and the C-terminal AMP. The 40-residue AMP is cationic, C-terminally amidated, and characterized by the presence of six cysteine molecules joined by three intramolecular disulfide bridges. Its cysteine array is common to another AMP and toxins from cnidarians; this suggests a common ancestor, as has been proposed for AMPs and toxins from arthropods. Damicornin was active in vitro against Gram-positive bacteria and the fungus Fusarium oxysporum. Damicornin expression was studied using a combination of immunohistochemistry, reverse phase HPLC, and quantitative RT-PCR. Our data show that damicornin is constitutively transcribed in ectodermal granular cells, where it is stored, and further released in response to nonpathogenic immune challenge. Damicornin gene expression was repressed by the coral pathogen Vibrio coralliilyticus. This is the first evidence of AMP gene repression in a host-Vibrio interaction.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Ecologie, systèmes aquatiques [036]
Localisation
Fonds IRD [F B010053634]
Identifiant IRD
fdi:010053634
