@article{fdi:010053621,
  title = {{L}aboratory selection for an accelerated mosquito sexual development rate},
  author = {{O}liva, {C}. {F}. and {B}enedict, {M}. {Q}. and {L}emp{\'e}ri{\`e}re, {G}uy and {G}illes, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {S}eparating males and females at the early adult stage did not ensure the virginity of females of {A}nopheles arabiensis ({D}ongola laboratory strain), whereas two years earlier this method had been successful. {I}n most mosquito species, newly emerged males and females are not able to mate successfully. {F}or anopheline species, a period of 24 h post-emergence is generally required for the completion of sexual maturation, which in males includes a 180 rotation of the genitalia. {I}n this study, the possibility of an unusually shortened sexual maturity period in the laboratory-reared colony was investigated. {M}ethods: {T}he effect of two different sex-separation methods on the virginity of females was tested: females separated as pupae or less than 16 h post-emergence were mated with males subjected to various doses of radiation. {T}-tests were performed to compare the two sex-separation methods. {T}he rate of genitalia rotation was compared for laboratory-reared and wild males collected as pupae in {D}ongola, {S}udan, and analysed by {Z}-tests. {S}permatheca dissections were performed on females mated with laboratory-reared males to determine their insemination status. {R}esults: {W}hen the sex-separation was performed when adults were less than 16 h post-emergence, expected sterility was never reached for females mated with radio-sterilized males. {E}xpected sterility was accomplished only when sexes were separated at the pupal stage. {O}bservation of genitalia rotation showed that some males from the laboratory strain {D}ongola were able to successfully mate only 11 h after emergence and 42% of the males had already completed rotation. {A} small proportion of the same age females were inseminated. {W}ild males showed a much slower genitalia rotation rate. {A}t 17 h post-emergence, 96% of the laboratory-reared males had completed genitalia rotation whereas none of the wild males had. {C}onclusion: {T}his colony has been cultured in the laboratory for over one hundred generations, and now has accelerated sexual maturation when compared with the wild strain. {T}his outcome demonstrates the kinds of selection that can be expected during insect colonization and maintenance, particularly when generations are non-overlapping and similar-age males must compete for mates.},
  keywords = {},
  booktitle = {},
  journal = {{M}alaria {J}ournal},
  volume = {10},
  numero = {},
  pages = {135},
  ISSN = {1475-2875},
  year = {2011},
  DOI = {10.1186/1475-2875-10-135},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053621},
}