@article{fdi:010053411,
  title = {{A} retrospective overview of enterovirus infection diagnosis and molecular epidemiology in the public hospitals of {M}arseille, {F}rance (1985-2005)},
  author = {{T}an, {C}. {Y}. {Q}. and {N}inove, {L}. and {G}audart, {J}. and {N}ougair{\`e}de, {A}. and {Z}andotti, {C}. and {T}hirion, {L}aurence and {C}harrel, {R}. and {D}e {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{H}uman enteroviruses ({HEV}) are frequent human pathogens and, associated in particular with large outbreaks of aseptic meningitis. {H}ere, we have compiled a database of clinical {HEV} isolates from the {P}ublic {H}ospitals of {M}arseille, from 1985 to 2005. {A}mongst 654 isolates that could be characterized by complete sequencing of the {VP}1 gene, 98% belonged to species {HEV}-{B}; the most frequently isolated serotypes were {E}chovirus {E}30, {E}11, {E}7, {E}6 and {E}4. {T}he high incidence of {E}30 and the recent emergence of {E}13 are consistent with reports worldwide and peak {HEV} isolation occurred mostly in the late spring and summer months. {T}he proportion of echoviruses has decreased across the years, while that of coxsackieviruses has increased. {S}tool (the most frequent sample type) allowed detection of all identified serotypes. {MRC}5 ({H}uman lung fibroblasts) cell line was the most conducive cell line for {HEV} isolation (84.9% of 10 most common serotype isolates, 96.3% in association with {BGM} ({B}uffalo green monkey kidney cells)). {P}revious seroneutralization-based serotype identification demonstrated 55.4% accuracy when compared with molecular {VP}1 analysis. {O}ur analysis of a large number of clinical strains over 20 years reinforced the validity of {VP}1 serotyping and showed that comparative p-distance scores can be coupled with phylogenetic analysis to provide non-ambiguous serotype identification. {P}hylogenetic analysis in the {VP}1, 2{C} and 3{D} regions also provided evidence for recombination events amongst clinical isolates. {I}n particular, it identified isolates with dissimilar {VP}1 but almost identical nonstructural regions.},
  keywords = {{FRANCE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {6},
  numero = {3},
  pages = {e18022},
  ISSN = {1932-6203},
  year = {2011},
  DOI = {10.1371/journal.pone.0018022},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053411},
}