@article{fdi:010053088,
  title = {{T}he '{PUCE} {CAFE}' {P}roject : the {F}irst 15{K} coffee microarray, a new tool for discovering candidate genes correlated to agronomic and quality traits},
  author = {{P}rivat, {I}. and {B}ardil, {A}. and {G}omez, {A}.{B}. and {S}everac, {D}. and {D}antec, {C}. and {F}uentes, {I}. and {M}ueller, {L}. and {J}o{\¨e}t, {T}hierry and {P}ot, {D}. and {F}oucrier, {S}. and {D}ussert, {S}t{\'e}phane and {L}eroy, {T}hierry and {J}ournot, {L}. and {K}ochko, {A}lexandre de and {C}ampa, {C}laudine and {C}ombes, {M}arie-{C}hristine and {L}ashermes, {P}hilippe and {B}ertrand, {B}enoit},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {U}nderstanding the genetic elements that contribute to key aspects of coffee biology will have an impact on future agronomical improvements for this economically important tree. {D}uring the past years, {EST} collections were generated in {C}offee, opening the possibility to create new tools for functional genomics. {R}esults: {T}he "{PUCE} {CAFE}" {P}roject, organized by the scientific consortium {NESTLE}/{IRD}/{CIRAD}, has developed an oligo-based microarray using 15,721 unigenes derived from published coffee {EST} sequences mostly obtained from different stages of fruit development and leaves in {C}offea {C}anephora ({R}obusta). {H}ybridizations for two independent experiments served to compare global gene expression profiles in three types of tissue matter (mature beans, leaves and flowers) in {C}. canephora as well as in the leaves of three different coffee species ({C}. canephora, {C}. eugenoides and {C}. arabica). {M}icroarray construction, statistical analyses and validation by {Q}-{PCR} analysis are presented in this study. {C}onclusion: {W}e have generated the first 15 {K} coffee array during this {PUCE} {CAFE} project, granted by {G}enoplante (the {F}rench consortium for plant genomics). {T}his new tool will help study functional genomics in a wide range of experiments on various plant tissues, such as analyzing bean maturation or resistance to pathogens or drought. {F}urthermore, the use of this array has proven to be valid in different coffee species (diploid or tetraploid), drastically enlarging its impact for high-throughput gene expression in the community of coffee research.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {G}enomics},
  volume = {12},
  numero = {},
  pages = {5},
  ISSN = {1471-2164},
  year = {2011},
  DOI = {10.1186/1471-2164-12-5},
  URL = {https://www.documentation.ird.fr/hor/fdi:010053088},
}