Demaneche S., Philippot L., David M. M., Navarro Elisabeth, Vogel T. M., Simonet P. (2009). Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach. Applied and Environmental Microbiology, 75 (2), p. 534-537. ISSN 0099-2240.
Titre du document
Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach
Demaneche S., Philippot L., David M. M., Navarro Elisabeth, Vogel T. M., Simonet P.
Source
Applied and Environmental Microbiology, 2009,
75 (2), p. 534-537 ISSN 0099-2240
We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters.
