@article{fdi:010049593,
  title = {{M}ultiple nuclear and mitochondrial genotyping identifies emperors and large-eye breams ({T}eleostei : {L}ethrinidae) from {N}ew {C}aledonia and reveals new large-eye bream species},
  author = {{B}orsa, {P}hilippe and {C}ollet, {A}deline and {C}arassou, {L}aure and {P}onton, {D}ominique and {C}hen, {W}. {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}pecies identification is fundamental to address questions about community ecology, biodiversity, conservation and resource management, at any life history stage. {C}urrent studies on fish larval ecology of tropical species are hampered by the lack of reliable and effective tools for identifying larvae at the species level. {E}mperors and large-eye breams comprise fish species from the perciform fish family {L}ethrinidae. {T}hey inhabit coastal and coral-reef habitats of the tropical {I}ndo-{P}acific, and they are important fishery resources. {T}heir taxonomy is considered difficult and identification to species is often problematic. {L}ethrinidae larvae and juveniles can be identified on the basis of meristic counts at the sub-family level, but no further. {I}n this study, we developed a set of polymorphic {PCR} markers (size polymorphisms at the intron regions from 4/5 nuclear protein-coding genes and single-strand conformation polymorphism of a 205-bp fragment at the mitochondrial 16{S} r{RNA} locus), to characterize 341 specimens from 21 {L}ethrinidae species from {N}ew {C}aledonia (southwestern tropical {P}acific). {A} genetic data-bank was constructed using the genotypes screened from the multiple gene loci of adult or sub-adult specimens used as references for these species. {T}he 16{S} r{RNA} gene fragment was able to differentiate species for the genus {L}ethrinus, but it provided little diagnostic resolution among different species within the genus {G}ymnocranius. {A} combination of the 16{S} r{RNA} marker and 4 nuclear markers developed herein allowed to sort out species within {G}ymnocranius spp. from {N}ew {C}aledonia. {U}sing genotype distributions at nuclear loci to test for reproductive isolation, we found that three apparently undescribed large-eye bream species may exist, provisionally referred to as {G}ymnocranius sp. {A}, sp. {B} and sp. {C}. {S}ubsequent genotyping of 137 {L}ethrinidae larvae collected from the bays of the {N}oumea peninsula, {N}ew {C}aledonia, found a total of three species ({L}ethrinus genivittatus, {L}ethrinus olivaceus and {G}ymnocranius sp. {A}).},
  keywords = {{F}ish ; {I}ndo-{P}acific ; {C}ryptic species ; {B}iological species concept ; {I}dentification ; {L}arvae ; {PCR} markers},
  booktitle = {},
  journal = {{B}iochemical {S}ystematics and {E}cology},
  volume = {38},
  numero = {3},
  pages = {370--389},
  ISSN = {0305-1978},
  year = {2010},
  DOI = {10.1016/j.bse.2010.03.007},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049593},
}