%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Roopesh, K.
%A Guyot, S.
%A Sabu, A.
%A Haridas, M.
%A Perraud Gaime, Isabelle
%A Roussos, Sevastianos
%A Augur, Christopher
%T Biotransformation of procyanidins by a purified fungal dioxygenase : identification and characterization of the products using mass spectrometry
%D 2010
%L fdi:010049513
%G ENG
%J Process Biochemistry
%@ 1359-5113
%K Procyanidins ; Aspergillus fumigatus ; Dioxygenase ; Purification ; Reversed-Phase High Performance Liquid ; Chromatography ; Electrospray ; Ionization-Mass Spectrometry
%M ISI:000277908100013
%N 6
%P 904-913
%R 10.1016/j.procbio.2010.02.019
%U https://www.documentation.ird.fr/hor/fdi:010049513
%> https://www.documentation.ird.fr/intranet/publi/2010/06-1/010049513.pdf
%V 45
%W Horizon (IRD)
%X Procyanidins commonly known as condensed tannins are a type of polyphenol with wide abundance naturally. They are commonly known as potent anti-oxidants with powerful free radical scavenging activity as well as anti-tumor-promoting activity. Little is known about the enzymatic mechanisms/pathways involved in the microbial biotransformation of these polyphenolic molecules. The extracellular enzyme, dioxygenase produced by Aspergillus fumigatus was used as in vitro tools to study the degradation pathway of a model procyanidin dimer, namely procyanidin B2. The enzyme was purified to homogeneity by a two step process of anion-exchange chromatography coupled with FPLC followed by gel-filtration chromatography coupled with HPLC and the molecular mass estimated. In addition, the different biotransformed products resulted from the dioxygenase action on P82 were purified using Reversed-Phase-High Performance Liquid Chromatography prior to their identification and characterization by structural elucidation using Electrospray Ionization-Mass Spectrometry. Subsequently, the mechanism of dioxygenase action on procyanidin dimer was defined.
%$ 084