@article{fdi:010049513,
  title = {{B}iotransformation of procyanidins by a purified fungal dioxygenase : identification and characterization of the products using mass spectrometry},
  author = {{R}oopesh, {K}. and {G}uyot, {S}. and {S}abu, {A}. and {H}aridas, {M}. and {P}erraud {G}aime, {I}sabelle and {R}oussos, {S}evastianos and {A}ugur, {C}hristopher},
  editor = {},
  language = {{ENG}},
  abstract = {{P}rocyanidins commonly known as condensed tannins are a type of polyphenol with wide abundance naturally. {T}hey are commonly known as potent anti-oxidants with powerful free radical scavenging activity as well as anti-tumor-promoting activity. {L}ittle is known about the enzymatic mechanisms/pathways involved in the microbial biotransformation of these polyphenolic molecules. {T}he extracellular enzyme, dioxygenase produced by {A}spergillus fumigatus was used as in vitro tools to study the degradation pathway of a model procyanidin dimer, namely procyanidin {B}2. {T}he enzyme was purified to homogeneity by a two step process of anion-exchange chromatography coupled with {FPLC} followed by gel-filtration chromatography coupled with {HPLC} and the molecular mass estimated. {I}n addition, the different biotransformed products resulted from the dioxygenase action on {P}82 were purified using {R}eversed-{P}hase-{H}igh {P}erformance {L}iquid {C}hromatography prior to their identification and characterization by structural elucidation using {E}lectrospray {I}onization-{M}ass {S}pectrometry. {S}ubsequently, the mechanism of dioxygenase action on procyanidin dimer was defined.},
  keywords = {{P}rocyanidins ; {A}spergillus fumigatus ; {D}ioxygenase ; {P}urification ; {R}eversed-{P}hase {H}igh {P}erformance {L}iquid ; {C}hromatography ; {E}lectrospray ; {I}onization-{M}ass {S}pectrometry},
  booktitle = {},
  journal = {{P}rocess {B}iochemistry},
  volume = {45},
  numero = {6},
  pages = {904--913},
  ISSN = {1359-5113},
  year = {2010},
  DOI = {10.1016/j.procbio.2010.02.019},
  URL = {https://www.documentation.ird.fr/hor/fdi:010049513},
}