@article{fdi:010048370,
  title = {{M}ultiplication and cryopreservation of vanilla ({V}anilla planifolia '{A}ndrews')},
  author = {{G}onzalez-{A}rnao, {M}. {T}. and {L}azaro-{V}allejo, {C}. {E}. and {E}ngelmann, {F}lorent and {G}amez-{P}astrana, {R}. and {M}artinez-{O}campo, {Y}. {M}. and {P}astelin-{S}olano, {M}. {C}. and {D}iaz-{R}amos, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} simple and efficient method for multiplication of vanilla ({V}anilla planifolia) was developed using in vitro fragmented explants ({IFE}s) as propagules. {IFE}s were obtained after dissecting apices from in vitro propagated clusters of plantlets, by cutting the remaining base of these plant clusters into segments of about 1 cm in length. {A}fter 4 months of culture on multiplication medium, 100% of {IFE}s produced up to 15 new shoots per explant, providing an efficient additional method for in vitro propagation of vanilla that maximizes the use of available material. {C}ryopreservation of apices from in vitro grown plants was achieved using the droplet vitrification protocol. {M}aximum survival (30%) and further regeneration (10%) of new shoots were obtained for apices derived from clusters of in vitro plantlets produced from microcuttings through a three-step droplet vitrification protocol: 1-d preculture of apices on solid {MS} medium with 0.3 {M} sucrose; loading with a 0.4 {M} sucrose + 2 {M} glycerol solution for 20-30 min; and exposure to plant vitrification solution {PVS}3 for 30 min at room temperature. {E}ven though the cryogenic protocol needs to be optimized to improve results, this work represents the first successful report of cryopreservation of vanilla apices.},
  keywords = {{M}ultiple shoots ; {A}pices ; {E}ncapsulation ; {V}itrification ; {D}roplet ; vitrification},
  booktitle = {},
  journal = {{I}n {V}itro {C}ellular and {D}evelopmental {B}iology-{P}lant},
  volume = {45},
  numero = {5},
  pages = {574--582},
  ISSN = {1054-5476},
  year = {2009},
  DOI = {10.1007/s11627-009-9242-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048370},
}