%0 Journal Article
%9 ACL : Articles dans des revues à comité de lecture répertoriées dans les bases internationales
%A Perera, P. I. P.
%A Yakandawala, D. M. D.
%A Hocher, Valérie
%A Verdeil, J. L.
%A Weerakoon, L. K.
%T Effect of growth regulators on microspore embryogenesis in coconut anthers
%D 2009
%L fdi:010048291
%J Plant Cell Tissue and Organ Culture
%K Androgenesis ; Anther culture ; Auxin ; Cytokinin ; Callus ; Cocos nucifera ; Embryo
%M ISI:000262088700007
%N 2
%P 171-180
%R 10.1007/s11240-008-9473-y
%U http://www.documentation.ird.fr/hor/fdi:010048291
%> http://www.documentation.ird.fr/intranet/publi/2009/compl2/010048291.pdf
%V 96
%W Horizon (IRD)
%X The effect of growth regulators on induction of androgenesis in coconut was investigated using seven different growth regulators at various concentrations and combinations. Three auxins (1-naphthalene acetic acid-NAA, indoleacetic acid-IAA, picloram) and three cytokinins (2-isopentyl adenine-2-iP, kinetin, zeatin) were tested either alone or in combination with 2,4-dichlorophenoxyacetic acid (2,4-D), using modified Eeuwens Y3 liquid medium as the basal medium. Among the tested auxins, 100 mu M NAA in combination with 100 mu M 2,4-D enhanced the production of calli/embryos (123) whereas IAA and picloram showed negative and detrimental effects, respectively, for androgenesis induction over 100 mu M 2,4-D alone. Kinetin and 2-iP enhanced the production of calli/embryos when 100 mu M 2,4-D was present in the culture medium. Both cytokinins at 10 mu M yielded the highest frequencies of embryos (113 and 93, respectively) whereas zeatin (1 or 2.5 mu M) had no impact on microspore embryogenesis. When calli/embryos (produced from different treatments in different experiments) were sub-cultured in somatic embryo induction medium (Y-3 medium containing 66 mu M 2,4-D), followed by maturation medium (Y-3 medium without growth regulators) and germination medium (Y-3 medium containing 5 mu M-6-benzyladenine-BA and 0.35 mu M gibberellic acid-GA(3)), plantlets were regenerated at low frequencies (in most treatments ranging from 0% to 7%).