Perera P. I. P., Vidhanaarachchi V. R. M., Gunathilake T. R., Yakandawala D. M. D., Hocher Valérie, Verdeil J. L., Weerakoon L. K. (2009). Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.). Plant Cell Tissue and Organ Culture, 99 (1), p. 73-81. ISSN 0167-6857.
Titre du document
Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.)
Perera P. I. P., Vidhanaarachchi V. R. M., Gunathilake T. R., Yakandawala D. M. D., Hocher Valérie, Verdeil J. L., Weerakoon L. K.
Source
Plant Cell Tissue and Organ Culture, 2009,
99 (1), p. 73-81 ISSN 0167-6857
Coconut is a cross pollinating palm, propagated only by seeds. Tissue culture is the only vegetative propagation method available for coconut. Consistent callogenesis was obtained by culturing unfertilised ovaries at -4 stage in CRI 72 medium containing 100 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1% activated charcoal. Callusing was improved by application of 9 mu M thidiazuron (TDZ). Embryogenic calli were subcultured onto somatic embryogenesis induction medium containing 66 mu M 2,4-D. Stunted growth was observed in the somatic embryos after subculture onto CRI 72 medium containing abscisic acid (ABA). Maturation of somatic embryos could be achieved in Y-3 medium without growth regulators. Conversion of somatic embryos was induced by adding gibberellic acid (GA(3)) to conversion medium containing 5 mu M 6-benzyladenine (BA) while 2-isopentyl adenine (2iP) increased the frequency of plant regeneration. A total of 83 plantlets was produced from 32 cultured ovaries.
