@article{fdi:010046353,
  title = {{D}engue 1 {D}iversity and {M}icroevolution, {F}rench {P}olynesia 2001-2006 : connection with {E}pidemiology and {C}linics},
  author = {{D}escloux, {E}. and {C}ao-{L}ormeau, {V}. {M}. and {R}oche, {C}. and de {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {D}engue fever ({DF}) is an emerging infectious disease in the tropics and subtropics. {D}eterminants of {DF} epidemiology and factors involved in severe cases-dengue haemorrhagic fever ({DHF}) and dengue shock syndrome ({DSS})-remain imperfectly characterized. {S}ince 2000, serotype 1 ({DENV}-1) has predominated in the {S}outh {P}acific. {T}he aim of this study was (i) to determine the origin and (ii) to study the evolutionary relationships of {DENV}-1 viruses that have circulated in {F}rench {P}olynesia ({FP}) from the severe 2001 outbreak to the recent 2006 epidemic, and (iii) to analyse the viral intra-host genetic diversity according to clinical presentation. {M}ethodology/{P}rincipal {F}indings: {S}equences of 181 envelope gene and 12 complete polyproteins of {DENV}-1 viruses obtained from human sera in {FP} during the 2001-2006 period were generated. {P}hylogenetic analysis showed that all {DENV}-1 {FP} strains belonged to genotype {IV}-"{S}outh {P}acific' and derived from a single introduction event from {S}outh-{E}ast {A}sia followed by a 6-year in situ evolution. {A}lthough the ratio of nonsynonymous/synonymous substitutions per site indicated strong negative selection, a mutation in the envelope glycoprotein ({S}222{T}) appeared in 2002 and was subsequently fixed. {I}t was noted that genetic diversification was very significant during the 2002-2005 period of endemic {DENV}-1 circulation. {F}or nine {DF} sera and eight {DHF}/{DSS} sera, approximately 40 clones/serum of partial envelope gene were sequenced. {I}mportantly, analysis revealed that the intra-host genetic diversity was significantly lower in severe cases than in classical {DF}. {C}onclusions/{S}ignificance: {F}irst, this study showed that {DENV}-1 epidemiology in {FP} was different from that described in other {S}outh-{P}acific islands, characterized by a long sustained viral circulation and the absence of new viral introduction over a 6-year period. {S}econd, a significant part of {DENV}-1 evolution was observed during the endemic period characterized by the rapid fixation of {S}222{T} in the envelope protein that may reflect genetic drift or adaptation to the mosquito vector. {T}hird, for the first time, it is suggested that clinical outcome may be correlated with intra-host genetic diversity.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {N}eglected {T}ropical {D}iseases},
  volume = {3},
  numero = {8},
  pages = {e493},
  ISSN = {1935-2735},
  year = {2009},
  DOI = {10.1371/journal.pntd.0000493},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046353},
}