%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Berling, M.
%A Blachère Lopez, C.
%A Soubabere, O.
%A Léry, Xavier
%A Bonhomme, A.
%A Sauphanor, B.
%A Lopez Ferber, M.
%T Cydia pomonella granulovirus genotypes overcome virus resistance in the codling moth and improve virus efficiency by selection against resistant hosts
%D 2009
%L fdi:010044252
%G ENG
%J Applied and Environmental Microbiology
%@ 0099-2240
%M ISI:000263119100005
%N 4
%P 925-930
%R 10.1128/aem.01998-08
%U https://www.documentation.ird.fr/hor/fdi:010044252
%> https://www.documentation.ird.fr/intranet/publi/2009/02/010044252.pdf
%V 75
%W Horizon (IRD)
%X Cydia pomonella granulovirus (CpGV) has been used for 15 years as a bioinsecticide in codling moth (Cydia pomonella) control. In 2004, some insect populations with low susceptibility to the virus were detected for the first time in southeast France. RGV, a laboratory colony of codling moths resistant to the CpGV-M isolate used in the field, was established with collection of resistant insects in the field followed by an introgression of the resistant trait into a susceptible colony (Sv). The resistance level (based on the 50% lethal concentrations [LC(50)s]) of the RGV colony to the CpGV-M isolate, the active ingredient in all commercial virus formulations in Europe, appeared to be over 60,000-fold compared to the Sv colony. The efficiency of CpGV isolates from various other regions was tested on RGV. Among them, two isolates (I12 and NPP-R1) presented an increased pathogenicity on RGV. I12 had already been identified as effective against a resistant C. pomonella colony in Germany and was observed to partially overcome the resistance in the RGV colony. The recently identified isolate NPP-R1 showed an even higher pathogenicity on RGV than other isolates, with an LC50 of 166 occlusion bodies (OBs)/mu l, compared to 1.36 x 10(6) OBs/mu l for CpGV-M. Genetic characterization showed that NPP-R1 is a mixture of at least two genotypes, one of which is similar to CpGV-M. The 2016-r4 isolate obtained from four successive passages of NPP-R1 in RGV larvae had a sharply reduced proportion of the CpGV-M-like genotype and an increased pathogenicity against insects from the RGV colony.
%$ 084