@article{fdi:010042654,
  title = {{C}onserved mosquito/parasite interactions affect development of {P}lasmodium falciparum in {A}frica - art. no. e1000069},
  author = {{M}endes, {A}. {M}. and {S}chlegelmilch, {T}. and {C}ohuet, {A}nna and {A}wono-{A}mbene, {P}. and {D}e {I}orio, {M}. and {F}ontenille, {D}idier and {M}orlais, {I}sabelle and {C}hristophides, {G}. {K}. and {K}afatos, {F}. {C}. and {V}lachou, {D}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n much of sub-{S}aharan {A}frica, the mosquito {A}nopheles gambiae is the main vector of the major human malaria parasite, {P}lasmodium falciparum. {C}onvenient laboratory studies have identified mosquito genes that affect positively or negatively the developmental cycle of the model rodent parasite, {P}. berghei. {H}ere, we use transcription profiling and reverse genetics to explore whether five disparate mosquito gene regulators of {P}. berghei development are also pertinent to {A}. gambiae/{P}. falciparum interactions in semi-natural conditions, using field isolates of this parasite and geographically related mosquitoes. {W}e detected broadly similar albeit not identical transcriptional responses of these genes to the two parasite species. {G}ene silencing established that two genes affect similarly both parasites: infections are hindered by the intracellular local activator of actin cytoskeleton dynamics, {WASP}, but promoted by the hemolymph lipid transporter, {A}po{II}/{I}. {S}ince {P}. berghei is not a natural parasite of {A}. gambiae, these data suggest that the effects of these genes have not been drastically altered by constant interaction and co-evolution of {A}. gambiae and {P}. falciparum; this conclusion allowed us to investigate further the mode of action of these two genes in the laboratory model system using a suite of genetic tools and infection assays. {W}e showed that both genes act at the level of midgut invasion during the parasite's developmental transition from ookinete to oocyst. {A}po{II}/{I} also affects the early stages of oocyst development. {T}hese are the first mosquito genes whose significant effects on {P}. falciparum field isolates have been established by direct experimentation. {I}mportantly, they validate for semi-field human malaria transmission the concept of parasite antagonists and agonists.},
  keywords = {},
  booktitle = {},
  journal = {{PL}o{S} {P}athogens},
  volume = {4},
  numero = {5},
  pages = {art. e1000069 [ 69]},
  ISSN = {1553-7366},
  year = {2008},
  DOI = {10.1371/journal.ppat.1000069},
  URL = {https://www.documentation.ird.fr/hor/fdi:010042654},
}