@article{fdi:010042560, title = {{C}oncentration and purification by magnetic separation of the erythrocytic stages of all human {P}lasmodium species - art. no. 45}, author = {{R}ibaut, {C}. and {B}erry, {A}. and {C}hevalley, {S}. and {R}eybier, {K}. and {M}orlais, {I}sabelle and {P}arzy, {D}. and {N}epveu, {F}ran{\c{c}}oise and {B}enoit {V}ical, {F}. and {V}alentin, {A}.}, editor = {}, language = {{ENG}}, abstract = {{B}ackground: {P}arasite concentration methods facilitate molecular, biochemical and immunological research on the erythrocytic stages of {P}lasmodium. {I}n this paper, an adaptation of magnetic {MACS}(({R})) columns for the purification of human {P}lasmodium species is presented. {T}his method was useful for the concentration/purification of either schizonts or gametocytes. {R}esults and conclusions: {T}he magnetic removal of non-parasitized red blood cells (in vivo and in vitro) using magnetic columns ( {MACS}) was evaluated. {T}his easy-to-use technique enriched schizonts and gametocytes from {P}lasmodium falciparum in vitro cultures with a very high degree of purity. {I}n addition, all haemozoin-containing stages (schizonts and/or gametocytes) from the peripheral blood of infected patients could be concentrated using this method. {T}his method is particularly useful for the concentration of non-falciparum species, which do not grow in culture and are otherwise difficult to obtain in large amounts.}, keywords = {}, booktitle = {}, journal = {{M}alaria {J}ournal}, volume = {7}, numero = {1}, pages = {{NIL}_1--{NIL}_5}, ISSN = {1475-2875}, year = {2008}, DOI = {10.1186/1475-2875-7-45}, URL = {https://www.documentation.ird.fr/hor/fdi:010042560}, }