A step-by-step tutorial to use HierFstat to analyse populations hierarchically structured at multiple levelsDe MeeûsThierryauteurautIRDGoudetJ.auteurauttextjournalArticletext/pdfborn digitalaccessThe populations of parasites and infectious agents are most of the time structured in complex hierarchy that lies beyond the classical nested design described by Wright's F-statistics (F-IS, F-ST and F-IT). In this note we propose a user-friendly step-by-step notice for using recent software (HierFstat) that computes and test fixation indices for any hierarchical structure. We add some tricks and tips for some special data kind (haploid, single focus), some other procedure (bootstrap over loci) and how to handle crossed factors.specializedfixation indicespopulation structurehierarchy052 020 Infection Genetics and Evolution7673173520071567-1348http://www.documentation.ird.fr/hor/fdi:01004090610.1016/j.meegid.2007.07.005IRD BondyF B010040906http://www.documentation.ird.fr/hor/fdi:010040906http://www.documentation.ird.fr/intranet/publi/2008/01/010040906.pdfAccès réservé (Intranet de l'IRD)IRD - Base Horizon / Pleins textes2008-02-222010-08-04fdi:010040906fre