Desquesnes M., Bosseno Marie-France, Brenière Simone F.. (2007). Detection of Chagas infections using Trypanosoma evansi crude antigen demonstrates high cross-reactions with Trypanosoma cruzi. Infection Genetics and Evolution, 7 (4), p. 457-462. ISSN 1567-1348.
Titre du document
Detection of Chagas infections using Trypanosoma evansi crude antigen demonstrates high cross-reactions with Trypanosoma cruzi
Desquesnes M., Bosseno Marie-France, Brenière Simone F.
Source
Infection Genetics and Evolution, 2007,
7 (4), p. 457-462 ISSN 1567-1348
Antigenic similarities between salivarian trypanosomes are known for a long time, but similarities between salivarian and stercorarian trypanosomes have been very little investigated. Phylogenetically, these genus and species appear to be far. However, in a preliminary work we had shown strong reactions of chagasic human sera using T evansi antigens in Western-blotting and ELISA. In the current work an ELISA test using T evansi crude antigens was probed with one hundred and two sera of chagasic Bolivian patients previously diagnosed which presented different pathologies. The sensitivity of the ELISA T evansi was 92.6% similar to that of ELISA T cruzi. The specificity evaluated using 20 sera of patients infected by Leishmania sp. reaches a comparable value of that obtained with the T cruzi immunofluorescent in in unotl uore scent assay. Finally, the sensitivity and the specificity of the ELISA T evansi were not really different from conventional serology of Chagas. In spite of their taxonomic position in various sections and their old divergence, these observations prove a strong antigenic community between T cruzi and T evansi. Consequently, the common antigens which remain to be characterized, could be an alternative source of antigen for the detection of antibodies against T cruzi. Given that T evansi seems to have strong antigenic communities with the majority of the pathogenic current trypanosomoses f mammals, it is very attractive to identify and characterize these highly conserved antigens which could be suitable targets to develop tools for diagnosis, prophylaxy and chemotherapy against several human and animal trypanosomoses.
