Virreira M., Truyens C., Alonso Vega C., Brutus Laurent, Jijena J., Torrico F., Carlier Yves, Svoboda M. (2007). Comparison of Trypanosoma cruzi lineages and levels of parasitic DNA in infected mothers and their newborns. American Journal of Tropical Medicine and Hygiene, 77 (1), p. 102-106. ISSN 0002-9637.
Titre du document
Comparison of Trypanosoma cruzi lineages and levels of parasitic DNA in infected mothers and their newborns
Virreira M., Truyens C., Alonso Vega C., Brutus Laurent, Jijena J., Torrico F., Carlier Yves, Svoboda M.
Source
American Journal of Tropical Medicine and Hygiene, 2007,
77 (1), p. 102-106 ISSN 0002-9637
To better understand the factors involved in maternal-fetal transmission of Trypanosoma cruzi, we compared DNA levels-obtained by use of quantitative real-time PCR and parasitic genotypes determined by PCR amplification followed by hybridization-in Bolivian mothers and their congenitally infected newborns. Mothers and their neonates displayed markedly different parasitic DNA levels, as most maternal estimated parasitemias (> 90%) were < 10 parasites/mL, whereas those of 76% of their newborns were > 1,000 parasites/mL. Comparison of T cruzi TeII sublineages infecting mothers and newborns showed identity, without evidence of mixed infection in mothers or neonates. Analysis of minor variants of TcIId-genotyped parasites using sequence class probes hybridizing with hypervariable domains of kDNA minicircles showed discrepancies in half of mother/newborn pairs.
