@article{fdi:010040634,
  title = {{IL}-12 producing monocytes and {IFN}-gamma and {TNF}-alpha producing {T}-lymphocytes are increased in placentas infected by {P}lasmodium falciparum},
  author = {{D}iouf, {I}brahima and {F}ievet, {N}adine and {D}oucour{\'e}, {S}ouleymane and {N}gom, {M}amadou and {A}ndrieu, {M}. and {M}athieu, {J}. {F}. and {G}aye, {A}. and {T}hiaw, {O}. {T}. and {D}eloron, {P}hilippe},
  abstract = {{P}lacental {P}lasmodium falciparum sequestration is associated with dysregulated immune function. {P}lacental inflammatory responses via {IFN}-gamma and {TNF}-alpha are implicated in functional damage. {H}owever, they are needed during placental infection to control asexual stage parasites. {T}o test the hypothesis that placental immunomodulation associated with malaria disturbs cytokine secretion differently in monocytes and lymphocytes, we have determined the proportion of monocytes and/or lymphocytes secreting {IFN}-gamma, {TNF}-alpha, {IL}-10 and {IL}-12. {I}ntervillous and peripheral blood monocyte ({CD}14(+)) and lymphocyte ({CD}3/{CD}4(+); {CD}3/{CD}8(+)) cytokine production was compared between 17 {P}. falciparum-infected and 12 non-infected {S}enegalese women. {A}fter culture with phorbolmyristate acetate/ionomycin ({PMA}/iono), lipopolysaccharide ({LPS}) or {P}. falciparum-infected erythrocytes ({IE}), the intracellular expression of cytokines in lymphocytes ({IFN}-gamma, {TNF}-alpha) and monocytes ({IL}-10, {IL}-12, {TNF}-alpha), was detected. {I}n response to {IE}, {CD}4(+) and {CD}8(+) {T}-cells produced {IFN}-gamma and {TNF}-alpha at similar rates in both compartments. {I}n response to {PMA}/iono, the frequencies of {CD}4(+) and {CD}8(+) {T}-cells producing {IFN}-gamma and {TNF}-alpha were similar in both compartments, but increased in {P}. falciparum-infected placentas. {I}n response to {LPS} or {IE}, {IL}-12 secreting monocytes were increased in infected women, while the frequency of {TNF}-alpha secreting monocytes was decreased compared to that in non-infected placenta. {T}he monocyte {IL}-12 response is not impaired in infected women. {IL}- 12 is an important factor for inducing {IFN}-gamma in {T}-cells. {T}hus, 1{L}-12 and {IFN}-alpha responses may synergistically allow a protective immune response in placental malaria. {TNF}-alpha production by {CD}4(+) and {CD}8(+) {T}-cells, is up-regulated in {P} falciparum-infected placentas, suggesting that {T}-cells actively participate to inflammatory responses.},
  keywords = {{P}lasmodium falciparum ; pregnancy ; malaria ; placenta ; {IL} 2 ; {TNF} alpha ; {INF} gamma},
  journal = {{J}ournal of {R}eproductive {I}mmunology},
  volume = {74},
  numero = {1-2},
  pages = {152--162},
  ISSN = {0165-0378},
  year = {2007},
  DOI = {10.1016/j.jri.2006.10.001},
  URL = {http://www.documentation.ird.fr/hor/fdi:010040634},
}