@article{fdi:010040630,
  title = {{R}ole of ethanol on growth, laccase production and protease activity in {P}ycnoporus cinnabarinus ss3},
  author = {{M}eza, {J}uan {C}arlos and {A}uria, {R}ichard and {L}omascolo, {A}. and {S}igoillot, {J}. {C}. and {C}asalot, {L}aurence},
  editor = {},
  language = {{ENG}},
  abstract = {{L}accase production by the strain {P}ycnoporus cinnabarinus ss3 was studied in a solid-state culture on sugar-cane bagasse using chemical compounds as inducers (ethanol, methanol, veratryl alcohol and ferulic acid). {L}accase productions were about 5- to 8.5-fold higher than non-induced cultures. {L}iquid-culture experiments with "{G}labeled ethanol were conducted. {N}inety-eight percent of the initial amount of {C}-14 from ethanol was recovered as ({CO}2)-{C}-14, {C}-14-biomass and soluble {C}-14-compounds (mainly ethanol). {T}he amount of {C}-14 in the biomass was only 6.8% of the total carbon consumed by {P} cinnabarinus, in absence of maltose, representing only 2.8% of added ethanol (1.1% and 1.6% in presence of maltose, respectively). {E}thanol was poorly used as carbon and energy sources for {P}. cinnabarinus growth and other carbon sources present in the liquid medium (yeast extract and sodium tartrate) were preferentially degraded. {T}ime-courses of laccase activity and gene expression were monitored in column in presence or in absence of ethanol vapors. {A}nalyses showed a perfect correlation between the activity and the amount of transcript. {A}fter 16 days of ethanol ventilation through the column, the ethanol flow was stopped. {I}mmediately, both laccase activity and gene expression decreased, but started to increase again as soon as the ventilation was restored. {I}n parallel, the effect of ethanol on protease activity in {R} cinnabarinus was measured. {P}resence of ethanol led to an inhibition of protease activity. {T}herefore ethanol plays a regulatory role on two elements (gene-expression and protease-activity levels) that are both in favor of an increase in laccase production by the fungus.},
  keywords = {laccase ; ethanol ; bagasse ; {P}ycnoporus cinnabarinus ; induction},
  booktitle = {},
  journal = {{E}nzyme and {M}icrobial {T}echnology},
  volume = {41},
  numero = {1-2},
  pages = {162--168},
  ISSN = {0141-0229},
  year = {2007},
  DOI = {10.1016/j.enzmictec.2006.12.018},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040630},
}