@article{fdi:010040601,
  title = {{T}he influence of desiccation and rehydration on the survival of polyembryonic seed of {C}itrus suhuiensis cv. limau madu},
  author = {{M}akeen, {M}. {A}. and {N}ormah, {M}. {N}. and {D}ussert, {S}t{\'e}phane and {C}lyde, {M}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} study was carried out to investigate the influence of desiccation and freezing followed by various presowing rehydration procedures on the desiccation sensitivity of the seed of {C}itrus suhuiensis cv. limau madu. {T}he freshly harvested seeds of limau madu were desiccated under a broad range of relative humidity ({RH}) to various equilibrium water contents (g {H}2{O} g(-1) dw). {T}he desiccated and desiccated-frozen seeds were either directly sown under germination conditions or subjected to presowing rehydration procedures: seed preheating, prehumidification and osmoconditioning. {T}he hydrated and desiccated seeds were sown in controlled germination conditions and the survival was evaluated 4-6 weeks after sowing. {T}he results showed that desiccation progressively reduced the percentage of normal seedling of the seeds of limau madu and the viability is almost lost at water contents below 0.08 g {H}2{O} g(-1) dw. {T}he estimated desiccation sensitivity was substantially high ({WC}50 = 0. 143 g {H}2{O} g(-1) dw) when the desiccated seeds were rapidly rehydrated (uncontrolled rehydration). {I}n contrast, seed prehumidification, preheating and osmoconditioning (controlled rehydration procedures) markedly enhanced normal seedling percentages decreasing the estimated values of {WC}50 (between 0.08 and 0. 127 g {H}2{O} g(-1) dw). {W}hile the rapidly rehydrated desiccated-frozen seeds were almost killed at water content of 0. 15 g {H}2{O} g(-1) dw, prehumidification and preheating have noticeably increased percentage of frozen seeds survival at the same water content. {H}owever, at water content of 0.21 g {H}2{O} g(-1) dw preheating significantly ({P} < 0.05) increased percentage of normal seedling of the frozen seeds. {S}eed desiccation markedly reduced the percentages of germinated seeds with multiple seedlings. {S}eed controlled rehydration remarkably increased the survival of polyembryos. {T}he beneficial effect of seed controlled rehydration on the survival of the desiccated seeds was pronounced at medium water contents (0.08-0.25 g {H}2{O} g(-1) dw).},
  keywords = {{C}itrus suhuiensis ; desiccation ; freezing ; rehydration},
  booktitle = {},
  journal = {{S}cientia {H}orticulturae},
  volume = {112},
  numero = {4},
  pages = {376--381},
  ISSN = {0304-4238},
  year = {2007},
  DOI = {10.1016/j.scienta.2006.12.050},
  URL = {https://www.documentation.ird.fr/hor/fdi:010040601},
}