%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Bodin, N.
%A Le Loc'h, François
%A Hily, C.
%T Effect of lipid removal on carbon and nitrogen stable isotope ratios in crustacean tissues
%D 2007
%L fdi:010037814
%G ENG
%J Journal of Experimental Marine Biology and Ecology
%@ 0022-0981
%K delta C 13 and delta N 15 ; amino acids ; chemical lipid extraction ; hepatopancreas ; Maja brachydactyla ; muscle
%M ISI:000244237300002
%N 2
%P 168-175
%R 10.1016/j.jembe.2006.09.008
%U https://www.documentation.ird.fr/hor/fdi:010037814
%> https://www.documentation.ird.fr/intranet/publi/2007/03/010037814.pdf
%V 341
%W Horizon (IRD)
%X The analysis of tissue's naturally occurring stable carbon and nitrogen isotope ratios is a useful tool to delineate trophic relationships. However, the interpretation of delta C-13 and delta N-15 is complicated by the influence of multiple factors such as the tissuespecific lipid content. The aim of this work was to evaluate the effects of lipid extraction on delta C-13 and delta N-15 compositions in muscle, hepatopancreas and gonads of a marine decapod crustacean, the spider crab Maja brachydacryla. Samples were analyzed for stable isotopes before and after lipid removal, using a derived Soxhlet extraction method. Differences in delta C-13 and delta N-15 were measured among tissues before and after treatment. Lipid extraction of muscle did not have a significant effect on either delta C-13 or delta N-15. By contrast, ecologically significant shifts forbothearbon and nitrogen stable isotopes ratios (+2.9 +/- 0.8 parts per thousand for delta C-13, and +1.2 +/- 0.7 parts per thousand for delta N-15) were noticed in the hepatopancreas. In regard to gonads, lipid extraction led to a shift only on delta C-13 (+1.3 +/- 0.3 parts per thousand). Finally, the derived Soxhlet extraction method removed the lipid influence for delta C-13, and had an effect on delta N-15 composition for lipid-rich samples. We recommend this treatment for carbon stable isotope studies on decapod crustacean lipid-rich tissues.
%$ 034