Telleria Jenny, Lafay Bénédicte, Virreira M., Barnabé Christian, Tibayrenc Michel, Svoboda M. (2006). Trypanosoma cruzi: Sequence analysis of the variable region of kinetoplast minicircles. Experimental Parasitology, 114 (4), p. 279-288. ISSN 0014-4894.
Titre du document
Trypanosoma cruzi: Sequence analysis of the variable region of kinetoplast minicircles
Telleria Jenny, Lafay Bénédicte, Virreira M., Barnabé Christian, Tibayrenc Michel, Svoboda M.
Source
Experimental Parasitology, 2006,
114 (4), p. 279-288 ISSN 0014-4894
The comparisons of 170 sequences of kinetoplast DNA minicircle hypervariable region obtained from 19 stocks of Trypanosoma cruzi and 2 stocks of Trypanosoma cruzi marenkellei showed that only 56% exhibited a significant homology one with other sequences. These sequences could be grouped into homology classes showing no significant sequence similarity with any other homology group. The 44% remaining sequences thus corresponded to unique sequences in our data set. In the DTU I ("Discrete Typing Units") 51% of the sequences were unique. In contrast, in the DTU IId, 87.5% of sequences were distributed into three classes. The results obtained for T. cruzi marinkellei, showed that all sequences were unique, without any similarity between them and T. cruzi sequences. Analysis of palindromes in all sequence sets show high frequency of the EcoRI site. Analysis of repetitive sequences suggested a common ancestral origin of the kDNA. The editing mechanism that occurs in kinetoplastidae is discussed.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
