Amevigbe M.D.D., Ferrer A., Champorie S., Monteny Nicole, Deunff J., Richard-Lenoble D. (2000). Isoenzymes of human lice : Pediculus humanus and P. capitis. Medical and Veterinary Entomology, 14 (4), p. 419-425. ISSN 0269-283X.
Titre du document
Isoenzymes of human lice : Pediculus humanus and P. capitis
Année de publication
2000
Auteurs
Amevigbe M.D.D., Ferrer A., Champorie S., Monteny Nicole, Deunff J., Richard-Lenoble D.
Source
Medical and Veterinary Entomology, 2000,
14 (4), p. 419-425 ISSN 0269-283X
Human lice (Phthiraptera : Pediculidae) from Africa, America and Europe were electrophoresed for 28 enzymes, with special interest in metabolic factors likely to be involved with insecticide resistance. Zymogram profiles of the body louse (Pediculus humanus L. from France and U.S.A.) and the head louse (P. capitis DeGeer from France, Madagascar, Mali & Senegal) were compared. Only two enzymes, phosphoglucomutase (Pgm) and esterase 3 (Est-3), showed electrophoretic variation. In our starch gel electrophoresis conditions, P. humanus showed three electromorphs of Pgm migrating anodally 6, 11 and 16 mm (designated alleles a, b, c, respectively). Of the putative Pgm alleles, b and c occured in all samples of both species of lice, whereas allele a was found only in P. humanus lab strain from U.S.A. Esterase 3 had four electromorphs migrating 23, 26, 30 and 35 mm (designated allele a, b, c and d). Among putative Est alleles, a was found only in P. capitis from Bamako (all 14 specimens aa homozygotes), allele d was found only in P. capitis from Dakar (39% frequency), whereas Est-3 alleles b and c showed apparently balanced polymorphism in all samples of both P. humanus and P. capitis except from Bamako. Despite the limited amount of isoenzyme variation detected (only 2/31 polymorphic loci), divergences of Est-3 and Pgm among Pediculus populations may be relevant to their biosystematics and resistance. (Résumé d'auteur)
Plan de classement
Méthodes de lutte, insecticides : résistance aux insecticides [052LUTIN03]
;
Divers [052TIAC05]
Descripteurs
POU ; ELECTROPHORESE ; ISOENZYME ; ESTERASE ; METHODE DE LUTTE ; INSECTICIDE ; SENSIBILITE RESISTANCE ; ETUDE EXPERIMENTALE ; PHOSPHOGLUCOMUTASE
Description Géographique
FRANCE ; MADAGASCAR ; MALI ; SENEGAL ; ETATS UNIS
Localisation
Fonds IRD [F B010024084] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010024084
