@inproceedings{fdi:010022398,
  title = {{E}valuation of {P}enicillium sp. {V}33{A}25 caffeinase activity in relation to its conservation method},
  author = {{G}utierrez-{S}anchez, {G}. and {G}aime {P}erraud, {I}sabelle and {A}ugur, {C}hristopher and {R}omano-{M}achado, {J}. and {S}aucedo-{C}astaneda, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}affeine degradation by {P}enicillium sp. strain {V}33{A}25 ({PV}33{A}25) was evaluated using a liquid culture medium prepared from a coffee infusion with an initial caffeine concentration of 0.5 g/{L}. {A}fter caffeine activity of the strain {PV}33{A}25 was induced, strain conservation was carried out by four methods : 1) conserved in a coffee-agar medium, 2) coffee pulp-agar medium, and for the next two methods, the spores were harvested from 3) potato dextrose agar ({PDA}) medium, and 4) coffee-agar. {T}he spores were lyophilized in the last two cases. {T}he strain was adapted three times by subculturing on coffee agar medium before being used for the evaluation of caffeine degradation ability. {I}t was observed that using methods 3 and 4 the fungi begin to degrade caffeine after 60 and 36 hours of culture, respectively. {N}evertheless, with method 1, the fungi starts to degrade caffeine 12 hours after the inoculation time, reaching 92% of caffeine degradation in a 72 hour period : with method 2, 39% of degradation was observed within 72 hours but it was necessary to make 6 precultures to reach a similar degradation rate with method 2. {T}he results of this study, suggest that the enzymatic activity responsible for caffeine degradation is inducible and once caffeine degradation ability is expressed, it is important to conserve the strain in a medium with caffeine. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{CAFE} ; {VALORISATION} {DE} {RESIDU} {AGROINDUSTRIEL} ; {ENSILAGE} ; {PULPE} ; {CAFEINE} ; {DEGRADATION} ; {FERMENTATION} {EN} {MILIEU} {SOLIDE} ; {CHAMPIGNON} ; {ENZYME} ; {INDUCTION} ; {ETUDE} {EXPERIMENTALE} ; {ETUDE} {COMPARATIVE} ; {MEXIQUE}},
  numero = {},
  pages = {373--376},
  booktitle = {{A}nais do 3 {S}eminario internacional sobre biotecnologia na agroindustria cafeeira = {P}roceedings of the 3rd international seminar on biotechnology in the coffee agroindustry},
  year = {2000},
  URL = {https://www.documentation.ird.fr/hor/fdi:010022398},
}