@article{fdi:010020874,
  title = {{C}hloroplast {DNA} extraction from herbaceous and woody plants for direct restriction fragment length polymorphism analysis},
  author = {{M}ariac, {C}edric and {T}rouslot, {P}ierre and {P}oteaux, {C}. and {B}ezan{\c{c}}on, {G}illes and {R}enno, {J}ean-{F}ran{\c{c}}ois},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he technique described here is a fast and simple method of extracting chloroplast {DNA} (cp{DNA}). {I}t overcomes the need for differential centrifugation using density gradients. {T}he leaves do not have to be kept in the dark and lyophilized before extraction, but lyophilization is still possible. {T}he chloroplasts are specifically lysed in a cell extract of leaves, using a non-ionic detergent. {A}fter isolation by centrifugation, the cp{DNA} is purified by the combined action of proteolytic enzymes and detergents, followed by the elimination of proteins using a mixture of chloroform and isoamyl alcohol. {T}his method provided good quality restriction profiles for all species analyzed. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{PLANTE} {HERBACEE} ; {ADN} ; {EXTRACTION} ; {POLYMORPHISME} ; {TECHNIQUE} {RFLP} ; {METHODOLOGIE} ; {ETUDE} {EXPERIMENTALE} ; {CHLOROPLASTE}},
  booktitle = {},
  journal = {{B}iotechniques},
  volume = {28},
  numero = {1},
  pages = {110; p. 111--113},
  ISSN = {0736-6205},
  year = {2000},
  URL = {https://www.documentation.ird.fr/hor/fdi:010020874},
}