Makowska Z., Keller J., Engelmann Florent. (1999). Cryopreservation of apices isolated from garlic (Allium sativum L.) bulbils and cloves. Cryo-Letters, 20 (3), p. 175-182. ISSN 0143-2044.
Titre du document
Cryopreservation of apices isolated from garlic (Allium sativum L.) bulbils and cloves
Année de publication
1999
Type de document
Article
Auteurs
Makowska Z., Keller J., Engelmann Florent
Source
Cryo-Letters, 1999,
20 (3), p. 175-182 ISSN 0143-2044
This paper reports on the successful application of the vitrification technique for cryopreserving apices sampled from bulbils and cloves of garlic. With bulbils, survival and regrowth of control apices were generally high after treatment with PVS2 and PVS3 vitrification solutions. However, after cryopreservation, apices treated with PVS3 solution had a much higher regrowth rate than those treated with PVS2 solution. An experiment performed with a total of 11 garlic accessions showed that the success of cryopreservation depended on the size of the bulbils from which apices had been dissected. The following trend was observed as regards regrowth of cryopreserved apices : the highest regrowth frequency was achieved with apices taken from large bulbils, whereas no regrowth was noted with apices from small bulbils, and regrowth of apices from medium-size bulbils was intermediate. Apices from cloves had higher survival and regrowth frequencies than those from bulbils. (Résumé d'auteur)
Plan de classement
Collection, conservation [076AMEPLA02]
;
Culture végétale in vitro [084VITRO]
