@article{fdi:010018102,
  title = {{P}opulation structure of the primary malaria vector in {S}outh {A}merica, {A}nopheles darlingi, using isozyme, random amplified polymorphic {DNA}, internal transcribed spacer 2, and morphologic markers},
  author = {{M}anguin, {S}ylvie and {W}ilkerson, {R}.{C}. and {C}onn, {J}.{E}. and {R}ubio-{P}alis, {Y}. and {D}anoff-{B}urg, {J}.{A}. and {R}oberts, {D}.{R}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} genetic and morphologic survey of #{A}nopheles darlingi$ populations collected from seven countries in {C}entral and {S}outh {A}merica was performed to clarify the taxonomic status of this major malaria vector species in the {A}mericas. {P}opulation genetics was based on three techniques including isozyme, random amplified polymorphic {DNA}-polymerase chain reaction ({RAPD}-{PCR}), and internal transcribed spacer 2 ({ITS}2) markers. {T}he results of the isozyme analysis indicated moderate differences in the allele frequencies of three putative loci (glutamate oxalaoacetate transaminase-1, isocitrate dehydrogenase-1, and phosphoglucomutase) of the 31 analyzed. {N}o fixed electromorphic differences separated the populations of #{A}n. darlingi$, which showed little genetic divergence ({N}ei distances = 0.976-0.995). {F}ragments produced by {RAPD}-{PCR} demonstrated evidence of geographic partitioning and showed that all populations were separated by small genetic distances as measured with the 1 - {S} distance matrix. {T}he {ITS}2 sequences for all samples were identical except for four individuals from {B}elize that differed by a three-base deletion ({CCC}). {T}he morphologic study demonstrated that the {E}uclidean distances ranged from 0.02 to 0.14, with the highest value observed between populations from {B}elize and {B}olivia. {B}ased on these analyses, all the #{A}n. darlingi$ populations examined demonstrated a genetic similarity that is consistent with the existence of a single species and suggest that gene flow is occurring throughout the species' geographic range. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{MOUSTIQUE} ; {VECTEUR} ; {STRUCTURE} {DE} {POPULATION} ; {TAXONOMIE} ; {MORPHOLOGIE} ; {STRUCTURE} {GENETIQUE} ; {TECHNIQUE} {PCR} ; {TECHNIQUE} {RAPD} ; {ISOENZYME} ; {MARQUEUR} {MOLECULAIRE} ; {MORPHOMETRIE} ; {MARQUEUR} {ITS}2 ; {AMERIQUE} {DU} {SUD} ; {AMERIQUE} {CENTRALE} ; {BELIZE} ; {BOLIVIE} ; {BRESIL}:{COLOMBIA} ; {PEROU} ; {VENEZUELA} ; {GUYANE} {FRANCAISE}},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {60},
  numero = {3},
  pages = {364--376},
  ISSN = {0002-9637},
  year = {1999},
  URL = {https://www.documentation.ird.fr/hor/fdi:010018102},
}