Padidam M., Beachy R.N., Fauquet Claude. (1999). A phage single-stranded DNA (ssDNA) binding protein complements ssDNA accumulation of a geminivirus and interferes with viral movement. Journal of Virology, 73 (2), p. 1609-1616. ISSN 0022-538X.
Titre du document
A phage single-stranded DNA (ssDNA) binding protein complements ssDNA accumulation of a geminivirus and interferes with viral movement
Année de publication
1999
Type de document
Article
Auteurs
Padidam M., Beachy R.N., Fauquet Claude
Source
Journal of Virology, 1999,
73 (2), p. 1609-1616 ISSN 0022-538X
Geminiviruses are plant viruses with circular single-stranded DNA (ssDNA) genomes encapsidated in double icosahedral particles. Tomato leaf curl geminivirus (ToLCV) requires coat protein (CP) for the accumulation of ssDNA in protopolasts and in plants but not for systemic infection and symptom development in plants. In the absence of CP, infected protoplasts accumulate reduced levels of ssDNA and increased amounts of double-stranded DNA (dsDNA), compared to accumulation in the presence of wild-type virus. To determine whether the gene 5 protein (g5p), an ssDNA binding protein from Escherichia coli phage M13, could restore the accumulation of ssDNA, ToLCV that lacked the CP gene was modified to express g5p or g5p fused to the N-terminal 66 amino acides of CP (CP66:6G:g5). The modified viruses led to the accumulation of wild-type levels of ssDNA. The high levels of dsDNA accumulation during infections with the modified viruses suggested a direct role for CP in viral DNA replication. ToLCV that produced the CP66:6G:g5 protein did not spread efficiently in Nicotiana benthamiana plants, and inoculated plants developed only very mild symptoms. In infected protoplasts, the CP66:6G:g5 protein was immunolocalized to nuclei. We propose that the fusion protein interferes with the function of the BV1 movement and thereby prevents spread of the infection. (Résumé d'auteur)
Plan de classement
Relations plante hôte-parasite / Lutte génétique (inclus le
[076MALPLA06]
