@article{fdi:010016787,
  title = {{R}egeneration of the tropical legume {A}eschynomene sensitiva {S}w. from root explants},
  author = {{C}ampa, {C}laudine and {C}haintreuil-{D}ongmo, {C}. and {D}reyfus, {B}ernard},
  editor = {},
  language = {{ENG}},
  abstract = {{R}egeneration of #{A}eschynomene sensitiva$ {S}w. after callogenesis was obtained form small (2-5 mm long) root explants of 30-day-old seedlings aseptically cultivated on {M}urashige and {S}koog medium supplemented with various concentrations of growth regulators. {A}fter 4 weeks, the best results were observed with 0.54 micro{M} alpha-naphthaleneacetic acid and 2.22 micro{M} benzyladenine. {O}n this medium, the rate of regeneration depended on seedling age and agar concentration. {T}he highest number of shoots per explant was obtained with small cuttings from 30-day-old seedlings grown on a medium containing 8 g/l of agar. {R}egeneration success was also dependent on explant size. {W}hen longer explants (7-20 mm) were cut from the main root, direct regeneration was obtained in two weeks. {T}hese cuttings also generated shoots through callogenesis in four weeks but always in lower quantities than with direct regeneration, whatever the seedling age. {H}ere also, the best regeneration was obtained with cuttings from 30-day-old seedlings maintained on a medium with 8 g/l of agar. {R}egenerants were rooted on growth-regulator-free {M}urashige and {S}koog medium and then acclimatized in a greenhouse. {A} better survival to transplantation was observed when plantlets were inoculated with the photosynthetic #{B}radyrhizobium$ strain {ORS} 278. {S}tem and root nodules developed on the inoculated plantlets and were able to fix nitrogen. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{CULTURE} {IN} {VITRO} ; {CALLOGENESE} ; {MILIEU} {DE} {CULTURE} ; {SYMBIOSE} ; {NODULE} {CAULINAIRE} ; {FIXATION} {BIOLOGIQUE} {DE} {L}'{AZOTE}},
  booktitle = {},
  journal = {{P}lant {C}ell, {T}issue and {O}rgan {C}ulture},
  volume = {44},
  numero = {0167-6857},
  pages = {149--154},
  year = {1996},
  DOI = {10.1007/{BF}00048193},
  URL = {https://www.documentation.ird.fr/hor/fdi:010016787},
}