@article{fdi:010015650,
  title = {{T}esticular structure, spermatogenesis and sperm cryopreservation in the {A}frican clariid catfish {H}eterobranchus longifilis ({V}alenciennes, 1840)},
  author = {{O}teme, {Z}iriga and {N}unez, {J}esus and {K}ouassi, {C}.{K}. and {H}em, {S}aurin and {A}gn{\`e}se, {J}ean-{F}ran{\c{c}}ois},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he morphological and physiological characteristics of the testes and the sperm of the catfish #{H}eterobranchus longifilis$ ({V}al.) are presented. {T}he effect of cryopreservation on the fertilizing capacity of the sperm was also evaluated. {T}esticular structure and spermatogenesis are described using histological techniques. {T}he coexistence in the lobules of spermatozoa and all the spermatogenic stages indicates that this species is able to perform continuous reproduction. {N}o seasonal trend was noticed in the evolution of the gonadosomatic index and in the quantity of the sperm produced over a year's period. {H}owever, maximum sperm production was observed in {A}pril and {S}eptember. {D}ifferent cryopreservation trials were conducted using a cryoprotective solution composed of 5% dimethylsulphoxide ({DMSO}), 5% glycerol, 10% hen's eggyolk and 80% {M}ounib's solution. {F}resh and cryopreserved semen gave equivalent hatching rates (81.1%, 83.4% and 78.9% respectively for the fresh, the 1-hour cryopreserved and the 8-month cryopreserved sperm). {P}ercentages of normal and deformed larvae were not affected by sperm cryopreservation. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{POISSON} ; {PHYSIOLOGIE} {ANIMALE} ; {APPAREIL} {REPRODUCTEUR} ; {MALE} ; {HISTOLOGIE} ; {CRYOCONSERVATION} ; {SPERME} ; {SPERMATOGENESE} ; {AFRIQUE}},
  booktitle = {},
  journal = {{A}quaculture {R}esearch},
  volume = {27},
  numero = {},
  pages = {805--813},
  ISSN = {1355-557{X}},
  year = {1996},
  DOI = {10.1111/j.1365-2109.1996.tb01239.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010015650},
}