@article{fdi:010015648,
  title = {{V}itellogenin receptors during vitellogenesis in the rainbow trout {O}ncorhynchus mykiss},
  author = {{N}unez, {J}esus and {B}on, {E}. and {L}e {M}enn, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ainbow trout vitellogenin receptors have been characterized by ligand blotting and {S}catchard analysis. {T}heir evolution has been studied over a reproductive cycle in a broodstock of 2-year-old females. {T}he receptors were prepared from ovarian membrane homogenates and were solubilized using n-octyl-beta-{D}-glucopyranoside. {T}he visualization of the receptor by ligand blotting using 125iodine-vitellogenin after sodium dodecyl sulfate electrophoresis revealed the existence of one major binding component corresponding to a protein of 113 k{D}a. {T}he {S}catchard transformation of the binding data revealed a single class of binding sites. {T}he variations of the binding characteristics ({K}d and maximum binding) were investigated during vitellogenesis. {T}his study revealed that the {K}d was not affected by oocyte growth during vitellogenesis, but was highly decreased in ovulated eggs. {T}he receptor number increased during the same period from 35 to 860 f{M} per oocyte, while the receptor number per mm2 of oocyte membrane surface was doubled during the same period. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{POISSON} {D}'{EAU} {DOUCE} ; {PHYSIOLOGIE} {ANIMALE} ; {REPRODUCTION} {SEXUEE} ; {FEMELLE} ; {CYCLE} {DE} {REPRODUCTION} ; {PROTEINE} ; {PURIFICATION} ; {ELECTROPHORESE} ; {VITELLOGENINE} ; {VITELLOGENESE}},
  booktitle = {},
  journal = {{T}he {J}ournal of {E}xperimental {Z}oology},
  volume = {274},
  numero = {},
  pages = {163--170},
  ISSN = {0022-104{X}},
  year = {1996},
  DOI = {10.1002/({SICI})1097-010{X}(19960215)274:3<163::{AID}-{JEZ}3>3.0.{CO};2-{M}},
  URL = {https://www.documentation.ird.fr/hor/fdi:010015648},
}