%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Lortet, Gilles
%A Méar, Nathalie
%A Lorquin, Jean
%A Dreyfus, Bernard
%A Lajudie, Philippe de
%A Rosenberg, C.
%A Boivin, Catherine
%T Nod factors thin-layer chromatography profiling as a tool to characterize symbiotic specificity of rhizobial strains : application to Sinorhizobium saheli, S. teranga, and Rhizobium sp. strains isolated from Acacia and Sesbania
%D 1996
%L fdi:010015493
%G ENG
%J Molecular Plant-Microbe Interactions
%@ 0894-0282
%K NODULE RACINAIRE ; FIXATION BIOLOGIQUE DE L'AZOTE ; PLANTE HOTE ; BACTERIE ; SOUCHE ; GENOME ; HYBRIDATION ; CHROMATOGRAPHIE
%N 8
%P 736-747
%U https://www.documentation.ird.fr/hor/fdi:010015493
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_7/b_fdi_51-52/010015493.pdf
%V 9
%W Horizon (IRD)
%X Rhizobia isolated from #Acacia$ or #Sesbania$ belong to several taxonomic groups, including the newly described species #Sinorhizobium saheli$, #Sinorhizobium teranga$, and the so-called cluster U. A collection of strains belonging to these different groups was analyzed in order to determine whether the host range of a strain could be correlated with various molecular nodulation determinants. Nodulation tests showed that, independently of their taxonomic position, all the strains isolated from the same plant genus exhibited a similar host range, which was different for #Sesbania$ and #Acacia$ isolates. The fact that #S. teranga$ strains nodulate either #Acacia$ or #Sesbania$ led us to subdivide this species into biovars #acaciae$ and #sesbaniae$. Thin-layer chromatography (TLC) analysis of the Nod factors synthesized by overproducing strains showed that strains isolated from the same plant genus exhibited similar TLC profiles and profiles of #Acacia$ and #Sesbania$ symbionts were easily distinguishable, #Acacia$ strains producing, in particular, sulfated molecules. In contrast, no correlation could be established between the host range of a strain and its plasmid content, the nature of the nod gene inducers or the presence of DNA sequences homologous to specific nod genes. We thus propose that Nod factor TLC profiling may be used as an easy and powerful tool for the classification of rhizobial strains on the basis of their symbiotic properties. (Résumé d'auteur)
%$ 084SYMBIO02