@article{fdi:010015493,
  title = {{N}od factors thin-layer chromatography profiling as a tool to characterize symbiotic specificity of rhizobial strains : application to {S}inorhizobium saheli, {S}. teranga, and {R}hizobium sp. strains isolated from {A}cacia and {S}esbania},
  author = {{L}ortet, {G}illes and {M}{\'e}ar, {N}athalie and {L}orquin, {J}ean and {D}reyfus, {B}ernard and {L}ajudie, {P}hilippe de and {R}osenberg, {C}. and {B}oivin, {C}atherine},
  editor = {},
  language = {{ENG}},
  abstract = {{R}hizobia isolated from #{A}cacia$ or #{S}esbania$ belong to several taxonomic groups, including the newly described species #{S}inorhizobium saheli$, #{S}inorhizobium teranga$, and the so-called cluster {U}. {A} collection of strains belonging to these different groups was analyzed in order to determine whether the host range of a strain could be correlated with various molecular nodulation determinants. {N}odulation tests showed that, independently of their taxonomic position, all the strains isolated from the same plant genus exhibited a similar host range, which was different for #{S}esbania$ and #{A}cacia$ isolates. {T}he fact that #{S}. teranga$ strains nodulate either #{A}cacia$ or #{S}esbania$ led us to subdivide this species into biovars #acaciae$ and #sesbaniae$. {T}hin-layer chromatography ({TLC}) analysis of the {N}od factors synthesized by overproducing strains showed that strains isolated from the same plant genus exhibited similar {TLC} profiles and profiles of #{A}cacia$ and #{S}esbania$ symbionts were easily distinguishable, #{A}cacia$ strains producing, in particular, sulfated molecules. {I}n contrast, no correlation could be established between the host range of a strain and its plasmid content, the nature of the nod gene inducers or the presence of {DNA} sequences homologous to specific nod genes. {W}e thus propose that {N}od factor {TLC} profiling may be used as an easy and powerful tool for the classification of rhizobial strains on the basis of their symbiotic properties. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{NODULE} {RACINAIRE} ; {FIXATION} {BIOLOGIQUE} {DE} {L}'{AZOTE} ; {PLANTE} {HOTE} ; {BACTERIE} ; {SOUCHE} ; {GENOME} ; {HYBRIDATION} ; {CHROMATOGRAPHIE}},
  booktitle = {},
  journal = {{M}olecular {P}lant-{M}icrobe {I}nteractions},
  volume = {9},
  numero = {8},
  pages = {736--747},
  ISSN = {0894-0282},
  year = {1996},
  URL = {https://www.documentation.ird.fr/hor/fdi:010015493},
}