%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Coste, J.
%A Montes, B.
%A Reynes, J.
%A Peeters, Martine
%A Segarra, C.
%A Vendrell, J.P.
%A Delaporte, Eric
%A Segondy, M.
%T Comparative evaluation of three assays for the quantitation of human immunodeficiency virus type 1 RNA in plasma
%D 1996
%L fdi:010014418
%G ENG
%J Journal of Medical Virology
%@ 0146-6615
%K SIDA ; VIRUS ; ARN ; METHODE D'ANALYSE ; ETUDE COMPARATIVE
%K VIH-1
%P 293-302
%R 10.1002/(SICI)1096-9071(199612)50:4<293::AID-JMV3>3.0.CO;2-3
%U https://www.documentation.ird.fr/hor/fdi:010014418
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_49-50/010014418.pdf
%V 50
%W Horizon (IRD)
%X Reverse transcriptase-coupled polymerase chain reaction (Amplicor HIV-1 Monitor), the branched DNA (bDNA) method (Quantiplex HIV-1 RNA) and the nucleic acid sequence-based assay (NASBA HIV-1 RNA QT) were comparatively evaluated for the quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in plasma. Among 60 plasma specimens from HIV-1 infected patients, HIV-1 RNA was detected in 56 by Amplicor (sensitivity, 93.3 %), in 41 by bDNA (sensitivity, 68.3 %), and in 60 by NASBA (sensitivity, 100 %). HIV-1 RNA was not detected by any of these methods in 34/34 plasma specimens from HIV-1-seronegative blood donors (specificity, 100 %). The HIV-1 RNA levels as determined by the different methods were correlated significantly. The frequency of concordant results (log difference less than 0.50) was 80.4 % between Amplicor and NASBA, 77.5 % between Amplicor and bDNA, and 58.6 % between bDNA and NASBA. After initiation of antiviral therapy, HIV-1 RNA level variations observed with the three methods were similar. HIV-1 RNA levels were inversely correlated with the CD4+ T cell counts, whereas no correlation was found with HIV-1 p24-antigen levels. When the methods were evaluated for reproducibility, coefficients of variation ranged from 11 % to 40 % for Amplicor, from 6 % to 35 % for bDNA, and from 13 % to 62 % for NASBA. Quantitation of HIV-1 RNA in culture supernatants from HIV-1 subtype A to H strains showed that bDNA can be used to quantitate RNA from all HIV-1 subtypes, whereas Amplicor failed to detect RNA from subtype A strains and NASBA subtype G strains. (Résumé d'auteur)
%$ 052MALTRA03