@article{fdi:010014418,
  title = {{C}omparative evaluation of three assays for the quantitation of human immunodeficiency virus type 1 {RNA} in plasma},
  author = {{C}oste, {J}. and {M}ontes, {B}. and {R}eynes, {J}. and {P}eeters, {M}artine and {S}egarra, {C}. and {V}endrell, {J}.{P}. and {D}elaporte, {E}ric and {S}egondy, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}everse transcriptase-coupled polymerase chain reaction ({A}mplicor {HIV}-1 {M}onitor), the branched {DNA} (b{DNA}) method ({Q}uantiplex {HIV}-1 {RNA}) and the nucleic acid sequence-based assay ({NASBA} {HIV}-1 {RNA} {QT}) were comparatively evaluated for the quantitation of human immunodeficiency virus type 1 ({HIV}-1) {RNA} in plasma. {A}mong 60 plasma specimens from {HIV}-1 infected patients, {HIV}-1 {RNA} was detected in 56 by {A}mplicor (sensitivity, 93.3 %), in 41 by b{DNA} (sensitivity, 68.3 %), and in 60 by {NASBA} (sensitivity, 100 %). {HIV}-1 {RNA} was not detected by any of these methods in 34/34 plasma specimens from {HIV}-1-seronegative blood donors (specificity, 100 %). {T}he {HIV}-1 {RNA} levels as determined by the different methods were correlated significantly. {T}he frequency of concordant results (log difference less than 0.50) was 80.4 % between {A}mplicor and {NASBA}, 77.5 % between {A}mplicor and b{DNA}, and 58.6 % between b{DNA} and {NASBA}. {A}fter initiation of antiviral therapy, {HIV}-1 {RNA} level variations observed with the three methods were similar. {HIV}-1 {RNA} levels were inversely correlated with the {CD}4+ {T} cell counts, whereas no correlation was found with {HIV}-1 p24-antigen levels. {W}hen the methods were evaluated for reproducibility, coefficients of variation ranged from 11 % to 40 % for {A}mplicor, from 6 % to 35 % for b{DNA}, and from 13 % to 62 % for {NASBA}. {Q}uantitation of {HIV}-1 {RNA} in culture supernatants from {HIV}-1 subtype {A} to {H} strains showed that b{DNA} can be used to quantitate {RNA} from all {HIV}-1 subtypes, whereas {A}mplicor failed to detect {RNA} from subtype {A} strains and {NASBA} subtype {G} strains. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{SIDA} ; {VIRUS} ; {ARN} ; {METHODE} {D}'{ANALYSE} ; {ETUDE} {COMPARATIVE} ; {VIH}-1},
  booktitle = {},
  journal = {{J}ournal of {M}edical {V}irology},
  volume = {50},
  numero = {},
  pages = {293--302},
  ISSN = {0146-6615},
  year = {1996},
  DOI = {10.1002/({SICI})1096-9071(199612)50:4<293::{AID}-{JMV}3>3.0.{CO};2-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010014418},
}