@article{fdi:010013312,
  title = {{B}acterioplankton production determined by {DNA} synthesis, protein synthesis, and frequency of dividing cells in {T}uamotu atoll lagoons and surrounding ocean},
  author = {{T}orr{\'e}ton, {J}ean-{P}ascal and {D}ufour, {P}hilippe},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his study compares three independent methods used for estimating bacterioplankton production in waters from the lagoon (mesotrophic) and the surrounding ocean (oligotrophic) of two atolls from the {T}uamotu archipelago ({F}rench {P}olynesia). {T}hymidine and leucine incorporation were calibrated in dilution cultures and gave consistent results when the first was calibrated against cell multiplication and the second against protein synthesis. {T}his study demonstrates that determining conversion factors strongly depends on the selected calculation method (modified derivative, integrative, and cumulative). {T}hese different estimates are reconciled when the very low proportion of active cells is accounted for. {F}requency of dividing-divided cells ({FDDC}) calibrated using the same dilution cultures led to unrealistically high estimates of bacterial production. {H}owever, highly significant correlations between {FDDC} and either thymidine- or leucine-specific incorporation per cell were found in lagoon waters in situ. {T}hese correlations became more positive when oceanic data were added. {T}his suggests that the {FDDC} method is also potentially valid to determine bacterioplankton growth rates after cross calibration with thymidine or leucine methods. {I}f recommended precautions are observed, the three methods tested in the present study would give reliable production estimates. ({R}{\'e}sum{\'e} d'auteur)},
  keywords = {{MICROPLANCTON} ; {BACTERIE} ; {LAGON} ; {OCEAN} ; {ABONDANCE} ; {BIOMASSE} ; {ACIDE} {AMINE} ; {PROTEINE} ; {ETUDE} {COMPARATIVE} ; {POLYNESIE} {FRANCAISE} ; {TUAMOTU} {GAMBIER}},
  booktitle = {},
  journal = {{M}icrobial {E}cology},
  volume = {32},
  numero = {},
  pages = {185--202},
  ISSN = {0095-3628},
  year = {1996},
  DOI = {10.1007/{BF}00185887},
  URL = {https://www.documentation.ird.fr/hor/fdi:010013312},
}