Roussos Sevastianos, Pyle D.L. (1998). Continuous enzymes and fungal metabolites production in solid state fermentation using a counter-current reactor. In :
Raimbault Maurice (ed.), Soccol C.R. (ed.), Chuzel G. (ed.). International training course on solid state fermentation. Montpellier : ORSTOM, p. 21-35 multigr. (Document ORSTOM Montpellier ; 98-1). Solid State Fermentation : International Training Course, Curitiba (BRA), 1997/10/06-10.
Titre du document
Continuous enzymes and fungal metabolites production in solid state fermentation using a counter-current reactor
Année de publication
1998
Type de document
Colloque
Auteurs
Roussos Sevastianos, Pyle D.L.
In
Raimbault Maurice (ed.), Soccol C.R. (ed.), Chuzel G. (ed.) International training course on solid state fermentation
Solid State Fermentation : International Training Course, Curitiba (BRA), 1997/10/06-10
This work presents the continuous production of fungal biomass and enzymes by solid state fermentation (SSF) in a counter-current reactor adapted for this purpose. Pre-germinated conidia of Aspergillus niger were used as an inoculum and sugarcane bagasse, embedded with a nutritive solution, was the solid support. The solids residence time distribution (RTD) was carried out by feeding one impulse of blue-coloured humidified bagasse and this RTD was fixed at 20 hours. This study demonstrated that the values of the measured parameters (pH, moisture, biomass, glucoamylase production) were similar to those reported for batch SSF using the same solid support and micro-organism. A marked increase in biomass occurred from the progressive compartment (from compartment no.1 to no.9) into the reactor and the enzyme production was important (40 IU/g dry exit solids). No mycelium damage or sporulation was observed. The above results confirmed that the continuous production of enzymes by SSF under no sterile conditions was successful. Inoculation with pre-germinated conidia shortened the processing time and allowed control of the age of the mycelium in each compartment. Aeration was accomplished by natural convection and moisture content had to be controlled. This process can be applied to the continuous production of fungal biomass and metabolites in SSF with industrial applications using environmentally friendly biotechnology. (Résumé d'auteur)
Plan de classement
Aérobies [084FERMEN01]
Descripteurs
FERMENTATION EN MILIEU SOLIDE ; MILIEU DE CULTURE ; PRODUCTION INDUSTRIELLE ; MICROORGANISME ; CHAMPIGNON ; ENZYME ; GERMINATION ; FERMENTEUR DE LABORATOIRE ; BAGASSE ; MYCELIUM ; GLUCOAMYLASE
