Dujardin Jean-Pierre, Torrez E.M., Le Pont François, Hervas D., Sossa D. (1997). Isozymic and metric variation in the Lutzomyia longipalpis complex. Medical and Veterinary Entomology, 11 (4), p. 394-400. ISSN 0269-283X.
Titre du document
Isozymic and metric variation in the Lutzomyia longipalpis complex
Année de publication
1997
Auteurs
Dujardin Jean-Pierre, Torrez E.M., Le Pont François, Hervas D., Sossa D.
Source
Medical and Veterinary Entomology, 1997,
11 (4), p. 394-400 ISSN 0269-283X
Male Lutzomyia longipalpis of two types from Bolivia were compared using isozyme electrophoresis and wing morphometry. One sample (ex Chiflonkaka Cave, alt. 2800 m at Toro Toro, Charcas Province, Potosi Department) was "two-spot" phenotype males (i.e. tergites III and IV with paired pale patches of pheromone glands), whereas two other locality samples (Apa Apa and Imanaco, Sud Yungas Province, La Paz Department) were one-spot male phenotype (only tergite IV with paired pale patches). Multilocus enzyme electrophoresis (using ACON, aGPD, GPI, IDH, MDH, ME, 6PGD, PGM, LAP and PEPB) found no difference between samples from adjacent hen houses at Apa Apa. Nei's standard genetic distance between one-spot samples Apa Apa and Imanaco (5 km apart, 1500 m alt.) was 0,001-0,002, whereas the two-spot males from Toro Toro (800 km away) showed a genetic distance of 0,081 from the one-spot males (Apa Apa and Imanaco). This genetic distance is commensurate with speciation, but may simply be intraspecific differentiation due to "isolation by distance". For comparative wing morphometry, we included additional material of one-spot males from Bolivia (Guyabal, Sud Yungas, La Paz), Brazil, Colombia and Nicaragua. These three other country samples were assumed to be different sibling species in the complex Lutzomyia longipalpis (Lanzaro et al., 1993). Statistics were based on univariate and multivariate analysis. The comparison between size-in and size-free canonical variate analysis (CVA) indicated that the wing morphometric divergence between one-spot and two-spot Bolivian phenotypes was not size dependent and could have taxonomic significance. (Résumé d'auteur)
Plan de classement
Répartition et bioécologie des phlébotomes [052PHLEIS01]
Descripteurs
LEISHMANIOSE ; VECTEUR ; PHENOTYPE ; VARIABILITE GENETIQUE ; ENZYME ; SPECIATION ; MORPHOTAXONOMIE ; ELECTROPHORESE
Description Géographique
BOLIVIE ; BRESIL ; COLOMBIE ; NICARAGUA
Localisation
Fonds IRD [F B010012239] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010012239
