Abol-Ela S., Khamis O., Giannotti J., Fédière Gilles, Léry Xavier, Belal M. (1996). Natural DNA-recombinations of Spodoptera littoralis NPV among field populations in Egypt. Bulletin of Faculty of Agriculture - University of Cairo, 47, p. 341-353. ISSN 0526-8613.
Titre du document
Natural DNA-recombinations of Spodoptera littoralis NPV among field populations in Egypt
Bulletin of Faculty of Agriculture - University of Cairo, 1996,
47, p. 341-353 ISSN 0526-8613
Six natural isolates belonging to Spodotera littoralis NPV were identified. Using the restriction endonucleases Eco RI and Bam HI, the DNA profiles of each isolate from Giza, Menya, Kaliubeia-Kaha, Kafr El-Sheikh, Gharbeia and Kaliubeia-Tokh were differentiated from that of the reference Egypt-84. DNA electrophoretic profiles on different isolates collected 5 years later were compared to the difference type. The DNA sequence was not changed trough three serial passages of viral isolates in vivo, while several viral clones were obtained after the 4th passage. (Résumé d'auteur)