%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture non répertoriées par l'AERES
%A Léry, Xavier
%A El-Tarras, A.
%A Monsarrat, Annie
%A Abol-Ela, S.
%A Giannotti, J.
%T Establishment of two cell lines from embryonic cells of Pectinophora gossypiella (Lepidoptera : Gelichiidae)
%D 1996
%L fdi:010012227
%G ENG
%J Applied Entomology and Zoology
%@ 0003-6862
%K INSECTE NUISIBLE ; COTON ; MILIEU DE CULTURE ; VIRUS ENTOMOPATHOGENE
%K CULTURE CELLULAIRE
%N 4
%P 557-565
%U https://www.documentation.ird.fr/hor/fdi:010012227
%> https://horizon.documentation.ird.fr/exl-doc/pleins_textes/pleins_textes_6/b_fdi_47-48/010012227.pdf
%V 31
%W Horizon (IRD)
%X Two cell lines from the most important insect pest of cotton, #Pectinophora gossypiella$ S., were obtained from an embryonic tissue culture. The cells were cultured in GRACE's modified medium. The first cell line, designated ORS-PgS (PgS), was obtained by selecting the non-adherent cells during subculturing and consisted of a homogeneous population of rounded cells. The second cell line, designated ORS-PgA (PgA), obtained without any artificial selection, was a heterogeneous population of spherical and spindle-shaped cells with a great capacity to adhere. The two cell lines had a doubling time of 40 and 48 h respectively and were sub-cultured for more than 50 passages. Their polypeptidic profiles appeared identical but were different from those of other lepidopteran cell lines tested. The two cell lines support the multiplication of #Autographa california$ nucleopolyhedrovirus, producing 100000 plaque forming units/ml. In 1 to 2% PgS cells, polyhedra were synthetized, whereas PgA cells multiplied only virions. (Résumé d'auteur)
%$ 076RAVPLA08 ; 020BIOL