Publications des scientifiques de l'IRD

Léry Xavier, El-Tarras A., Monsarrat Annie, Abol-Ela S., Giannotti J. (1996). Establishment of two cell lines from embryonic cells of Pectinophora gossypiella (Lepidoptera : Gelichiidae). Applied Entomology and Zoology, 31 (4), p. 557-565. ISSN 0003-6862.

Titre du document
Establishment of two cell lines from embryonic cells of Pectinophora gossypiella (Lepidoptera : Gelichiidae)
Année de publication
1996
Type de document
Article
Auteurs
Léry Xavier, El-Tarras A., Monsarrat Annie, Abol-Ela S., Giannotti J.
Source
Applied Entomology and Zoology, 1996, 31 (4), p. 557-565 ISSN 0003-6862
Two cell lines from the most important insect pest of cotton, Pectinophora gossypiella S., were obtained from an embryonic tissue culture. The cells were cultured in GRACE's modified medium. The first cell line, designated ORS-PgS (PgS), was obtained by selecting the non-adherent cells during subculturing and consisted of a homogeneous population of rounded cells. The second cell line, designated ORS-PgA (PgA), obtained without any artificial selection, was a heterogeneous population of spherical and spindle-shaped cells with a great capacity to adhere. The two cell lines had a doubling time of 40 and 48 h respectively and were sub-cultured for more than 50 passages. Their polypeptidic profiles appeared identical but were different from those of other lepidopteran cell lines tested. The two cell lines support the multiplication of Autographa california nucleopolyhedrovirus, producing 100000 plaque forming units/ml. In 1 to 2% PgS cells, polyhedra were synthetized, whereas PgA cells multiplied only virions. (Résumé d'auteur)
Plan de classement
Biologie [020BIOL] ; Lutte biologique / Lutte intégrée [076RAVPLA08]
Descripteurs
INSECTE NUISIBLE ; COTON ; MILIEU DE CULTURE ; VIRUS ENTOMOPATHOGENE ; CULTURE CELLULAIRE
Localisation
Fonds IRD [F B010012227]
Identifiant IRD
fdi:010012227
Contact